Shahan K, Denaro M, Gilmartin M, Shi Y, Derman E
Mol Cell Biol. 1987 May;7(5):1947-54. doi: 10.1128/mcb.7.5.1947-1954.1987.
Mouse major urinary proteins (MUPs) are encoded by a family of about 35 to 40 highly conserved genes. In the preceding paper (K. Shahan, M. Gilmartin, and E. Derman, Mol. Cell. Biol. 7:1938-1946, 1987), we presented the sequences of the most abundant MUP mRNAs in the liver (MUP I, II, and III) and in the lachrymal (MUP IV) and submaxillary (MUP V) glands. We have shown that these five mRNAs are coded by five distinct genes, MUP I through V. In the present communication, we examine the expression of MUP genes in all of the six tissues in which MUP mRNAs are synthesized, the mammary, parotid, sublingual, lachrymal, and submaxillary glands and the liver. We show that gene MUP II is expressed in the liver and in the mammary gland, that gene MUP IV is expressed in the lachrymal and parotid glands, and that gene MUP V is expressed in the submaxillary, sublingual, and lachrymal and parotid glands, and that gene MUP V is expressed in the submaxillary, sublingual, and lachrymal glands. Furthermore, we present evidence that in addition to genes MUP I through V, another gene, MUP VI, is expressed in BALB/c mice in the parotid gland. The tissue-specific synthesis of MUP mRNAs is thus brought about by two major mechanisms: the expression, in different tissues, of different members of the family and the expression of a single gene at various levels in different tissues. When a particular MUP gene is expressed in several tissues, transcripts of this gene initiate at the same site and are spliced and polyadenylated in the same manner.
小鼠主要尿蛋白（MUPs）由一个约35至40个高度保守基因的家族编码。在前一篇论文（K. 沙汉、M. 吉尔马丁和E. 德曼，《分子与细胞生物学》7:1938 - 1946，1987年）中，我们展示了肝脏中最丰富的MUP mRNA（MUP I、II和III）以及泪腺（MUP IV）和颌下腺（MUP V）中的序列。我们已经表明这五种mRNA由五个不同的基因MUP I至V编码。在本通讯中，我们研究了MUP基因在合成MUP mRNA的所有六种组织中的表达情况，即乳腺、腮腺、舌下腺、泪腺、颌下腺和肝脏。我们发现基因MUP II在肝脏和乳腺中表达，基因MUP IV在泪腺和腮腺中表达，基因MUP V在颌下腺、舌下腺、泪腺和腮腺中表达。此外，我们提供证据表明，除了基因MUP I至V之外，另一个基因MUP VI在BALB/c小鼠的腮腺中表达。因此，MUP mRNA的组织特异性合成是由两种主要机制实现的：该家族不同成员在不同组织中的表达以及单个基因在不同组织中以不同水平的表达。当一个特定的MUP基因在多个组织中表达时，该基因的转录本在同一位置起始，并以相同方式进行剪接和聚腺苷酸化。
