Fetchick D A, Bertolini D R, Sarin P S, Weintraub S T, Mundy G R, Dunn J F
J Clin Invest. 1986 Aug;78(2):592-6. doi: 10.1172/JCI112614.
The human T cell lymphotrophic virus type I (HTLV-I) has recently been identified in a T cell lymphoma associated with hypercalcemia and increased bone turnover. Since increased serum concentrations of 1,25-dihydroxyvitamin D have been reported in this disease, we have examined the capacity of HTLV-I-infected cord blood lymphocytes to metabolize 25-hydroxyvitamin D3. Our results demonstrate that HTLV-I-infected cells have the capacity to metabolize 25-hydroxyvitamin D3 to a substance that co-migrates with 1,25-dihydroxyvitamin D3 by high performance liquid chromatography over a silica column using either 12% isopropanol in hexane or 5% isopropanol in dichloromethane. The metabolite binds to the 1,25-dihydroxyvitamin D3 receptor in rat osteosarcoma cells and stimulates bone resorption in cultures of fetal rat long bones. Mass spectrometric analysis of the metabolite confirmed the presence of 1,25-dihydroxyvitamin D3. Production of 1,25-dihydroxyvitamin D by lymphoma cells may contribute to the pathogenesis of the hypercalcemia seen in patients with HTLV-I-associated T cell lymphomas.
人类嗜T细胞病毒I型（HTLV-I）最近在一例与高钙血症和骨转换增加相关的T细胞淋巴瘤中被发现。由于该疾病中已报道血清1,25-二羟维生素D浓度升高，我们检测了感染HTLV-I的脐血淋巴细胞代谢25-羟维生素D3的能力。我们的结果表明，感染HTLV-I的细胞有能力将25-羟维生素D3代谢为一种物质，在使用正己烷中12%异丙醇或二氯甲烷中5%异丙醇的硅胶柱上进行高效液相色谱分析时，该物质与1,25-二羟维生素D3共迁移。该代谢产物与大鼠骨肉瘤细胞中的1,25-二羟维生素D3受体结合，并刺激胎鼠长骨培养物中的骨吸收。对该代谢产物的质谱分析证实了1,25-二羟维生素D3的存在。淋巴瘤细胞产生1,25-二羟维生素D可能有助于HTLV-I相关T细胞淋巴瘤患者高钙血症的发病机制。
