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正钒酸盐及类似含氧阴离子在欧洲鳗鲡(Anguilla anguilla)离体肠道中的运输与盐和水运输的关系。

The transport of orthovanadate and similar oxyanions in relation to salt and water transport across the isolated intestine of the common eel, Anguilla anguilla.

作者信息

Bell M V, Kelly K F, Sargent J R

出版信息

J Exp Biol. 1983 Jan;102:295-305. doi: 10.1242/jeb.102.1.295.

DOI:10.1242/jeb.102.1.295
PMID:6550622
Abstract

An intestinal preparation from the common eel (Anguilla anguilla) was characterized for both mucosal to serosal and serosal to mucosal transport of water, alanine, sodium and chloride. The preparation showed a net outflux of water and a net influx of alanine, and no net fluxes of sodium or chloride. The influxes of all four substances and the outflux of water were inhibited by ouabain, while the outfluxes of alanine, sodium and chloride were increased by ouabain. Influxes and outfluxes of the oxyanions orthovanadate, phosphate, arsenate, sulphate, selenate and chromate were measured over a range of concentrations from 10(-8) M to 10(-4) M. The entry of each anion was dependent on the mucosal concentration, not saturable within the concentration range used or competitive with chloride. There were no net fluxes of these anions and pertechnecate from solutions containing equal mucosal and serosal concentrations of the anion.

摘要

对欧洲鳗鲡(Anguilla anguilla)的肠道制剂进行了表征,以研究水、丙氨酸、钠和氯从黏膜到浆膜以及从浆膜到黏膜的转运情况。该制剂显示水有净外流,丙氨酸有净内流,而钠或氯没有净通量。哇巴因抑制了所有四种物质的内流和水的外流,而哇巴因增加了丙氨酸、钠和氯的外流。在10⁻⁸ M至10⁻⁴ M的浓度范围内测量了原钒酸盐、磷酸盐、砷酸盐、硫酸盐、硒酸盐和铬酸盐等含氧阴离子的内流和外流。每种阴离子的进入取决于黏膜浓度,在所使用的浓度范围内不饱和,也不与氯竞争。对于含有相等黏膜和浆膜浓度阴离子的溶液,这些阴离子和锝酸盐没有净通量。

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