Okamoto H, Greenbaum L M
Life Sci. 1983 Apr 25;32(17):2007-13. doi: 10.1016/0024-3205(83)90052-8.
Studies have compared "total", HMW kininogen and leukokininogen levels in human, rabbit and rat plasma using trypsin, glass powder and cathepsin D as kininogenases or activators of kininogenases. Rat plasma was found to have about 10 fold more leukokininogen than the other plasmas assayed. When trypsin was used to estimate total kininogen, rat plasma liberated maximal amounts of kinin only in the presence of high concentrations of trypsin (1 mg/ml incubation mixture). In addition, it was found that trypsin in these concentrations liberated from rat plasma both bradykinin and a previously unidentified kinin which we have termed "T-kinin". The results overall indicate that in the case of rat and rabbit plasma, currently used methods for estimations of total kininogen may not be accurate. T-kinin may represent a leukokininogen or a hitherto undescribed kininogen.
研究使用胰蛋白酶、玻璃粉和组织蛋白酶D作为激肽原酶或激肽原酶激活剂，比较了人、兔和大鼠血浆中的“总”、高分子量激肽原和白细胞激肽原水平。发现大鼠血浆中的白细胞激肽原比其他被测血浆多约10倍。当使用胰蛋白酶估计总激肽原时，大鼠血浆仅在高浓度胰蛋白酶（1mg/ml孵育混合物）存在下释放最大量的激肽。此外，还发现这些浓度的胰蛋白酶从大鼠血浆中释放出缓激肽和一种以前未鉴定的激肽，我们将其称为“T-激肽”。总体结果表明，对于大鼠和兔血浆，目前用于估计总激肽原的方法可能不准确。T-激肽可能代表一种白细胞激肽原或一种迄今未描述的激肽原。
