Thistlethwaithe P, Davidson D D, Fidler I J, Roth J A
Cancer Immunol Immunother. 1983;15(1):11-6. doi: 10.1007/BF00199455.
An enzyme-linked immunoassay (ELISA) was developed to study syngeneic humoral immune response to a primary tumor and its metastases in the K-1735 ultraviolet light (UV)-induced C3H murine melanoma system. Binding of sera from syngeneic animals previously immunized with primary tumor or metastatic tumor tissue (M-3, M-4) to corresponding 3 M KCl extracts of tumor was significantly greater than binding of control C3H mouse serum. Antibody binding was not significantly reduced by competitive binding with syngeneic murine muscle or liver extracts, indicating the presence of tumor antigen(s) not shared by normal murine tissue. Antibodies to the tumor-associated antigens were selectively removed by competitive binding with syngeneic K-1735 tumor extracts but not by the unrelated 102 murine sarcoma from C57BL/6. However, EL-4 extracts (C57BL/6) did inhibit antibody binding to the primary and both metastases. Further competitive binding studies demonstrated the presence of a common antigen(s) present on the primary tumor and both metastases. We conclude that the K-1735 UV-induced melanoma primary tumor and its metastases express serologically detectable shared antigenic determinate.
开发了一种酶联免疫吸附测定法（ELISA），以研究同基因小鼠对K - 1735紫外线（UV）诱导的C3H小鼠黑色素瘤系统中原发性肿瘤及其转移灶的体液免疫反应。先前用原发性肿瘤或转移性肿瘤组织（M - 3、M - 4）免疫的同基因动物血清与相应肿瘤的3M KCl提取物的结合，明显大于对照C3H小鼠血清的结合。与同基因小鼠肌肉或肝脏提取物竞争性结合后，抗体结合未显著降低，表明存在正常小鼠组织不共有的肿瘤抗原。与同基因K - 1735肿瘤提取物竞争性结合可选择性去除肿瘤相关抗原的抗体，但与来自C57BL/6的无关102小鼠肉瘤提取物竞争性结合则不能。然而，EL - 4提取物（C57BL/6）确实抑制了抗体与原发性肿瘤和两种转移灶的结合。进一步的竞争性结合研究表明，原发性肿瘤和两种转移灶上存在共同抗原。我们得出结论，K - 1735紫外线诱导的黑色素瘤原发性肿瘤及其转移灶表达血清学上可检测到的共同抗原决定簇。
