Brüggen J, Sorg C
Cancer Immunol Immunother. 1983;15(3):200-5. doi: 10.1007/BF00199165.
The hybridoma technique was used to generate monoclonal antibodies against a wide spectrum of melanoma-associated surface antigens. Mice were immunized against the human melanoma lines Mel A-375, SK Mel-25, and Mel S-5 (subclone of SK Mel-25), which differ with respect to a number of biological and biochemical properties. Spleen cells were fused with P3 X 63-AG8.653 myeloma cells. Twenty hybridomas producing antibodies that were negative on platelets, leukocytes, and monocytes but positive on melanoma cells were isolated and recloned. The specificity of antibodies was investigated on 30 human melanoma and nonmelanoma lines. Five groups of antibodies could be distinguished by their reactivity (1) with few melanoma lines and embryonic fibroblasts; (2) with melanoma, neuroblastoma, and teratoma; (3) with melanoma, neuroblastoma, glioblastoma, teratoma, and carcinoma; (4) with melanoma, teratoma, and carcinoma; and (5) with melanoma, neuroblastoma, teratoma, glioblastoma, carcinoma, embryonic fibroblasts, and B-lymphoblastoid cells. The antigen expression was qualitatively and quantitatively different from cell line to cell line. No evidence for melanoma-specific antigens was found. Eight antibodies were isolated detecting phenotypic differences on sublines of SK Mel-25.
采用杂交瘤技术制备针对多种黑色素瘤相关表面抗原的单克隆抗体。用人类黑色素瘤细胞系Mel A - 375、SK Mel - 25和Mel S - 5（SK Mel - 25的亚克隆）免疫小鼠，这些细胞系在一些生物学和生化特性方面存在差异。将脾细胞与P3 X 63 - AG8.653骨髓瘤细胞融合。分离并再次克隆出20个杂交瘤，其产生的抗体在血小板、白细胞和单核细胞上呈阴性，但在黑色素瘤细胞上呈阳性。在30个人类黑色素瘤和非黑色素瘤细胞系上研究抗体的特异性。根据抗体的反应性可区分出五组抗体：（1）与少数黑色素瘤细胞系和胚胎成纤维细胞反应；（2）与黑色素瘤、神经母细胞瘤和畸胎瘤反应；（3）与黑色素瘤、神经母细胞瘤、胶质母细胞瘤、畸胎瘤和癌反应；（4）与黑色素瘤、畸胎瘤和癌反应；（5）与黑色素瘤、神经母细胞瘤、畸胎瘤、胶质母细胞瘤、癌、胚胎成纤维细胞和B淋巴细胞样细胞反应。不同细胞系的抗原表达在定性和定量方面均存在差异。未发现黑色素瘤特异性抗原的证据。分离出8种抗体，可检测SK Mel - 25亚系的表型差异。
