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天然溶菌酶精确完整的抗原结构。

The precise and entire antigenic structure of native lysozyme.

作者信息

Atassi M Z, Lee C L

出版信息

Biochem J. 1978 May 1;171(2):429-34. doi: 10.1042/bj1710429.

DOI:10.1042/bj1710429
PMID:656054
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1183972/
Abstract

The exact boundary, residue, conformational and directional definitions of the three antigenic sites of native hen's egg-white lysozyme are described. The results clearly reveal that the three antigenic sites account quantitatively for the total antigenic reactivity of the protein. Thus the entire antigenic structure of lysozyme has now been precisely determined and is briefly discussed here, together with the power of the surface-stimulation synthetic concept.

摘要

描述了天然鸡蛋清溶菌酶三个抗原位点的确切边界、残基、构象和方向定义。结果清楚地表明，这三个抗原位点在数量上占该蛋白质总抗原反应性的比例。因此，溶菌酶的整个抗原结构现已精确确定，并在此简要讨论，同时还讨论了表面刺激合成概念的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74c6/1183972/2a82981d781c/biochemj00488-0150-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74c6/1183972/b4f2e4775c06/biochemj00488-0149-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74c6/1183972/2a82981d781c/biochemj00488-0150-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74c6/1183972/b4f2e4775c06/biochemj00488-0149-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74c6/1183972/2a82981d781c/biochemj00488-0150-a.jpg

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本文引用的文献

1
Immunochemistry of sperm whale myoglobin. IV. The role of the arginine residues in the conformation and differentiation of their roles in the antigenic reactivity.抹香鲸肌红蛋白的免疫化学。IV. 精氨酸残基在其构象及抗原反应性中不同作用的分化里的作用。
Biochemistry. 1969 Aug;8(8):3385-94. doi: 10.1021/bi00836a037.
2
Immunochemistry of sperm whale myoglobin. I. The specific interaction of some tryptic peptides and of peptides containing all the reactive regions of the antigen.抹香鲸肌红蛋白的免疫化学。I. 一些胰蛋白酶肽段以及包含抗原所有反应区域的肽段的特异性相互作用。
Biochemistry. 1968 Feb;7(2):688-98. doi: 10.1021/bi00842a026.
3
A major part of the polypeptide chain of tobacco mosaic virus protein is antigenic.
烟草花叶病毒蛋白的多肽链的主要部分具有抗原性。
EMBO J. 1985 May;4(5):1231-5. doi: 10.1002/j.1460-2075.1985.tb03765.x.
4
Design of peptide enzymes (pepzymes): surface-simulation synthetic peptides that mimic the chymotrypsin and trypsin active sites exhibit the activity and specificity of the respective enzyme.肽酶（pepzyme）的设计：模拟胰凝乳蛋白酶和胰蛋白酶活性位点的表面模拟合成肽表现出相应酶的活性和特异性。
Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8282-6. doi: 10.1073/pnas.90.17.8282.
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The sequence-immunology correlation revisited: data for cetacean myoglobins and mammalian lysozymes.再探序列与免疫学的相关性：鲸类肌红蛋白和哺乳动物溶菌酶的数据
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10
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Proc R Soc Lond B Biol Sci. 1967 Apr 18;167(1009):365-77. doi: 10.1098/rspb.1967.0034.