针对代表溶菌酶抗原位点的表面模拟肽的抗体与溶菌酶的结合。
Binding with lysozyme of antibodies against surface-simulation peptides representing the lysozyme antigenic sites.
作者信息
Atassi M Z, Sakata S
出版信息
Biochem J. 1982 Mar 1;201(3):669-72. doi: 10.1042/bj2010669.
Abstract
Previously it had been shown that native lysozyme has three discontinuous antigenic sites (comprising spatially adjacent residues that may be distant in sequence) that were mimicked by surface-simulation synthetic peptides that had the capacity to bind the bulk (97-99%) of the antibody response against native lysozyme. In the present work these three surface-simulation synthetic peptides were coupled to succinoylated bovine serum albumin, and the conjugates were injected into rabbits. Antibodies against each peptide reacted, as expected, only with that peptide, but it was also found that the antibodies could bind with lysozyme, and the complete specificity of this binding was rigorously established. The advantages of these findings in conformational and immunological investigations are outlined.
摘要
先前已经表明，天然溶菌酶有三个不连续的抗原位点（由空间上相邻但序列上可能相距较远的残基组成），这些位点被表面模拟合成肽所模拟，这些合成肽能够结合针对天然溶菌酶的大部分（97 - 99%）抗体反应。在本研究中，将这三种表面模拟合成肽与琥珀酰化牛血清白蛋白偶联，并将偶联物注射到兔子体内。针对每种肽的抗体如预期那样仅与该肽发生反应，但还发现这些抗体能够与溶菌酶结合，并且严格确定了这种结合的完全特异性。概述了这些发现在构象和免疫学研究中的优势。
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Periodate oxidation of sperm-whale myoglobin and the role of the methionine residues in the antigen-antibody reaction.抹香鲸肌红蛋白的高碘酸盐氧化作用以及甲硫氨酸残基在抗原 - 抗体反应中的作用。
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