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布氏锥虫糖体（微体）中烷氧基磷脂生物合成起始步骤的定位

Localization of the initial steps in alkoxyphospholipid biosynthesis in glycosomes (microbodies) of Trypanosoma brucei.

作者信息

Opperdoes F R

出版信息

FEBS Lett. 1984 Apr 9;169(1):35-9. doi: 10.1016/0014-5793(84)80284-7.

DOI:10.1016/0014-5793(84)80284-7
PMID:6562019
Abstract

Cell fractionation of Trypanosoma brucei cultured procyclic stages showed that the key enzyme of glycerol-ether lipid synthesis, dihydroxyacetone-phosphate acyltransferase (EC 2.3.1.42) was exclusively associated with the microbody fraction. These organelles contained in addition 1-acyl glycerol-3-phosphate: NADP+ oxidoreductase (EC 1.1.1.101) and acyl-CoA reductase and were capable of utilizing DHAP, but not G-3-P, as substrate for lysophosphatidic acid formation. It is concluded that in T. brucei the glycosomes are the exclusive site of the synthesis of precursors for glycerol-ether lipid synthesis and that they contain the entire pathway to form alkoxylipids from glycerol and acyl-CoA.

摘要

对布氏锥虫培养的前循环期进行细胞分级分离显示，甘油醚脂合成的关键酶——磷酸二羟丙酮酰基转移酶（EC 2.3.1.42）仅与微体部分相关联。这些细胞器还含有1-酰基甘油-3-磷酸：NADP⁺氧化还原酶（EC 1.1.1.101）和酰基辅酶A还原酶，并且能够利用磷酸二羟丙酮（DHAP）而非甘油-3-磷酸（G-3-P）作为溶血磷脂酸形成的底物。得出的结论是，在布氏锥虫中，糖体是甘油醚脂合成前体的唯一合成部位，并且它们包含从甘油和酰基辅酶A形成烷氧基脂质的完整途径。

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