Hewitt H B, Blake E R
Br J Cancer. 1978 May;37(5):718-22. doi: 10.1038/bjc.1978.109.
Two murine lymphomas of spontaneous origin were stored in liquid N2 as cell suspensions in DMSO for 8 and 13 years respectively. Isogenic transplantation assays done some years before freezing and immediately after thawing indicated no measurable loss of clonogenic cells during freezing, storage or thawing; the number of cells required for 50% successful transplantation remained close to unity in both cases. The overall data revealed no evidence of an alteration of the receptivity of the mouse colonies over a period of 13 or 20 years. We attribute this remarkable stability to close supervision of the systems within a single laboratory.
两个自发产生的小鼠淋巴瘤分别作为细胞悬液保存在含有二甲基亚砜（DMSO）的液氮中8年和13年。在冷冻前几年和解冻后立即进行的同基因移植试验表明，在冷冻、储存或解冻过程中，克隆细胞没有可测量的损失；在这两种情况下，50%成功移植所需的细胞数量都保持接近一致。总体数据显示，在13年或20年的时间里，没有证据表明小鼠群体的接受性发生了改变。我们将这种显著的稳定性归因于在单一实验室对这些系统的密切监控。
