小鼠心脏中mRNA的发育调控

Developmental regulation of mRNA in mouse heart.

作者信息

Ouellette A J, Croall D E, Van Ness J, Ingwall J S

出版信息

Proc Natl Acad Sci U S A. 1983 Jan;80(1):223-7. doi: 10.1073/pnas.80.1.223.

DOI:10.1073/pnas.80.1.223

PMID:6571996

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC393344/

Abstract

The myocardium contains abundant translatable mRNAs that change during development. Maximal cell-free synthesis of [3H]leucine-, [35S]methionine-, and [35S]cysteine-labeled translation products directed by poly(A)-containing mRNAs from 12-, 14-, and 17-day fetal; 5-day-old neonatal; and 30-day-old adult mouse heart was determined by using one- and two-dimensional polyacrylamide gels. Three general developmental patterns of heart-specific mRNA translation products were observed: two translatable mRNAs were most abundant in 12-day fetal heart; five mRNAs were most abundant in 14- and 17-day fetal heart and occurred only at low concentrations in 12-day fetal and adult heart; four mRNAs, including mRNAs coding for actin, tropomyosin, and myosin light chains 1 and 2, were most abundant in the adult heart. Thus, differentiating cardiac muscle is characterized by a complex pattern of mRNA regulation.

摘要

心肌含有丰富的可翻译mRNA，这些mRNA在发育过程中会发生变化。通过使用一维和二维聚丙烯酰胺凝胶，测定了来自12天、14天和17天胎儿、5天大的新生儿以及30天大的成年小鼠心脏的含poly(A)mRNA指导的[3H]亮氨酸、[35S]甲硫氨酸和[35S]半胱氨酸标记的翻译产物的无细胞合成最大值。观察到心脏特异性mRNA翻译产物的三种一般发育模式：两种可翻译mRNA在12天胎儿心脏中最为丰富；五种mRNA在14天和17天胎儿心脏中最为丰富，而在12天胎儿和成年心脏中仅以低浓度出现；四种mRNA，包括编码肌动蛋白、原肌球蛋白以及肌球蛋白轻链1和2的mRNA，在成年心脏中最为丰富。因此，分化中的心肌的特征是mRNA调控的复杂模式。