Jurecka W, Lassmann H, Hörandner H
Cell Tissue Res. 1978 May 29;189(2):305-12. doi: 10.1007/BF00209279.
The proliferative activity of newborn and adult mouse adrenal medullary cells was determined with light and electron microscopic autoradiography. The H3 thymidine labelling index of 2 weeks old mice adrenal medullary cells was about 9.4% and declined to less than 1% in adult mice. In electron microscopic autoradiography labelled norepinephrine as well as epinephrine cells could be seen. Only in 1 and 2 weeks old mice some morphologically undifferentiated cells were visible. In formaldehyde induced fluorescence combined with light microscopic autoradiography the fluorescence intensities of labelled and unlabelled medullary cells were measured. On average the fluorescence intensity of labelled cells was lower than that of unlabelled cells. The differences could be explained by a higher number of autoradiographic silver grains laying on the cytoplasm of labelled cells. These results give evidence that fully differentiated adrenal medullary cells are capable of division.
采用光镜和电镜放射自显影术测定新生和成年小鼠肾上腺髓质细胞的增殖活性。2周龄小鼠肾上腺髓质细胞的H3胸腺嘧啶核苷标记指数约为9.4%，成年小鼠则降至1%以下。在电镜放射自显影中，可以看到标记的去甲肾上腺素能细胞和肾上腺素能细胞。仅在1周龄和2周龄小鼠中可见一些形态未分化的细胞。在甲醛诱导荧光结合光镜放射自显影中，测定了标记和未标记髓质细胞的荧光强度。平均而言，标记细胞的荧光强度低于未标记细胞。这些差异可以用标记细胞细胞质上较多的放射自显影银粒来解释。这些结果表明，完全分化的肾上腺髓质细胞具有分裂能力。
