Biegel J A, Boggs S S, Conner M K
Cancer Res. 1982 Jul;42(7):2813-5.
The recent development of an AKR(Rb6.15)1Ald lymphoma model in our laboratory presents a unique means of comparing sister chromatid exchange (SCE) frequencies and cellular replication kinetics of normal and lymphoma cells in the same host environment. Lymphoma cells, distinguished from normal cells by the presence of two metacentric chromosomes, had an average cell cycle time of 8 hr compared to 11 hr for normal cells. Normal bone marrow cells from nonleukemic AKR/J, nonleukemic AKR(Rb6.15) Ald, and tumor passage recipients had similar baseline and 1,3-bis(2-chloroethyl)-1-nitrosourea-induced SCE frequencies following exposure to 4.4 mg 1,3-bis(2-chloroethyl)-1-nitrosourea per kg and 19 hr 5-bromo-2-deoxyuridine. While baseline SCE frequencies were only slightly higher, 1,3-bis(2-chloroethyl)-1-introsourea-induced lymphoma cell SCE frequencies were 3 times higher than normal induced SCE frequencies [34.06 +/- 6.6 (S.E.) versus 11.0 +/- 0.6 SCEs/cell].
我们实验室最近开发的AKR(Rb6.15)1Ald淋巴瘤模型提供了一种独特的方法，可在同一宿主环境中比较正常细胞和淋巴瘤细胞的姐妹染色单体交换(SCE)频率及细胞复制动力学。淋巴瘤细胞通过两条中着丝粒染色体与正常细胞区分开来，其平均细胞周期时间为8小时，而正常细胞为11小时。来自非白血病AKR/J、非白血病AKR(Rb6.15)Ald和肿瘤传代受体的正常骨髓细胞，在每千克暴露于4.4毫克1,3-双(2-氯乙基)-1-亚硝基脲和19小时5-溴-2-脱氧尿苷后，具有相似的基线以及1,3-双(2-氯乙基)-1-亚硝基脲诱导的SCE频率。虽然基线SCE频率仅略高，但1,3-双(2-氯乙基)-1-亚硝基脲诱导的淋巴瘤细胞SCE频率比正常诱导的SCE频率高3倍[34.06±6.6(标准误)对11.0±0.6次SCE/细胞]。
