Naito K, Yamaguchi H, Horibe K, Shiku H, Takahashi T, Suzuki S, Yamada K
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2341-5. doi: 10.1073/pnas.80.8.2341.
Serum from a patient (CO) with acute lymphoblastic leukemia was reactive in immunoadherence assays with autologous leukemia cells but not with autologous blood lymphocytes or bone marrow cells during complete remission. Extensive absorption tests with an array of leukemia cells and normal cells were performed in order to define the specificity of the reaction. The autologous leukemia reactivity was either completely or partially absorbed with acute lymphoblastic leukemia cells obtained from 10 of 14 patients but not with acute nonlymphoblastic leukemia cells or normal hematopoietic cells. Some cultured lymphocytic leukemia lines also showed expression of the antigen. The absence of alloantibodies in CO's serum detectable in our assay allowed direct testing of CO's serum on allogeneic target cells. The results of these tests correlated with the results of absorption tests, indicating that small populations of antigen-positive cells were not missed with absorption tests. In addition, absorption tests with CO-positive allogeneic leukemia target cells showed the same pattern of antigen distribution as tests with autologous targets. CO reactivity was not removed by absorption with cell lines expressing human T-cell leukemia virus, nor did CO's serum react with adult T-cell leukemia cells from three patients.
一名急性淋巴细胞白血病患者（CO）的血清在免疫黏附试验中与自体白血病细胞发生反应，但在完全缓解期与自体血淋巴细胞或骨髓细胞不发生反应。为了确定反应的特异性，对一系列白血病细胞和正常细胞进行了广泛的吸收试验。自体白血病反应性被14例患者中10例的急性淋巴细胞白血病细胞完全或部分吸收，但未被急性非淋巴细胞白血病细胞或正常造血细胞吸收。一些培养的淋巴细胞白血病系也显示出该抗原的表达。在我们的检测中未检测到CO血清中的同种抗体，这使得可以直接用CO血清检测异基因靶细胞。这些检测结果与吸收试验结果相关，表明吸收试验没有遗漏少量抗原阳性细胞。此外，用CO阳性异基因白血病靶细胞进行的吸收试验显示出与用自体靶细胞进行的试验相同的抗原分布模式。用表达人类T细胞白血病病毒的细胞系吸收不能去除CO反应性，CO的血清也不与三名患者的成人T细胞白血病细胞发生反应。
