Shau H, Koren H S, Dawson J R
Br J Cancer. 1983 May;47(5):687-95. doi: 10.1038/bjc.1983.107.
Natural killing (NK) by lymphocytes from normal individuals against primary and established ovarian carcinoma cell lines was tested in short-term chromium release assays. Two established cell lines and 5/6 primary cell lines tested showed significant susceptibility to NK. Primary cell lines are, in general, less sensitive to NK than long-term cultured target cells. A common NK recognition determinant on ovarian carcinoma cells and on the erythroleukaemic K562 cells was demonstrated by cold target inhibition assays. The recognition structure was also present on an ovarian cell line resistant to NK but not on the insensitive leukaemic cell line, SB. The activity against ovarian carcinoma cells was associated with the presence of large granular lymphocytes (LGL) previously shown to be the major effector cells against K562 targets. In fractions obtained by Percoll gradient centrifugation of lymphocytes, only fractions with a high content of LGL demonstrated good NK activity. LGL mediated NK against both non-adherent K562 and the adherent ovarian carcinoma target cells independent of monocytes.
在短期铬释放试验中，检测了正常个体淋巴细胞对原发性和已建立的卵巢癌细胞系的自然杀伤（NK）作用。所检测的两个已建立的细胞系和5/6原发性细胞系对NK表现出显著的敏感性。一般来说，原发性细胞系对NK的敏感性低于长期培养的靶细胞。通过冷靶抑制试验证明，卵巢癌细胞和红白血病K562细胞上存在共同的NK识别决定簇。该识别结构也存在于对NK有抗性的卵巢细胞系上，但不存在于不敏感的白血病细胞系SB上。针对卵巢癌细胞的活性与大颗粒淋巴细胞（LGL）的存在有关，此前已证明LGL是针对K562靶标的主要效应细胞。在通过淋巴细胞的Percoll梯度离心获得的组分中，只有LGL含量高的组分表现出良好的NK活性。LGL介导对非贴壁K562和贴壁卵巢癌靶细胞的NK作用，且不依赖于单核细胞。
