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嗜热脂肪芽孢杆菌的卡那霉素抗性突变体，其缺乏对16S核糖体RNA中两个相邻腺苷进行甲基化的酶。

Kasugamycin resistant mutants of Bacillus stearothermophilus lacking the enzyme for the methylation of two adjacent adenosines in 16S ribosomal RNA.

作者信息

Van Buul C P, Damm J B, Van Knippenberg P H

出版信息

Mol Gen Genet. 1983;189(3):475-8. doi: 10.1007/BF00325912.

DOI:10.1007/BF00325912
PMID:6575236
Abstract

Several mutants of B. stearothermophilus have been isolated that are resistant to the antibiotic kasugamycin. One of these is shown to lack dimethylation of two adjacent adenosines in the 16S ribosomal RNA. All mutants that were analyzed biochemically lack the enzyme that is able to methylate this site. Ribosomal sensitivity and resistance to kasugamycin in B. stearothermophilus is therefore, like in E. coli, closely connected with dimethylation of the adenosines.

摘要

已分离出几种嗜热栖热菌的突变体，它们对抗生素春日霉素具有抗性。其中一种突变体显示在16S核糖体RNA中两个相邻腺苷缺乏二甲基化。所有经过生化分析的突变体都缺乏能够使该位点甲基化的酶。因此，嗜热栖热菌中核糖体对春日霉素的敏感性和抗性，与大肠杆菌一样，与腺苷的二甲基化密切相关。

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Kasugamycin resistant mutants of Bacillus stearothermophilus lacking the enzyme for the methylation of two adjacent adenosines in 16S ribosomal RNA.嗜热脂肪芽孢杆菌的卡那霉素抗性突变体，其缺乏对16S核糖体RNA中两个相邻腺苷进行甲基化的酶。
Mol Gen Genet. 1983;189(3):475-8. doi: 10.1007/BF00325912.
2
Nucleotide sequence of the ksgA gene of Escherichia coli: comparison of methyltransferases effecting dimethylation of adenosine in ribosomal RNA.大肠杆菌ksgA基因的核苷酸序列：影响核糖体RNA中腺苷二甲基化的甲基转移酶比较。
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Studies on the function of two adjacent N6,N6-dimethyladenosines near the 3' end of 16 S ribosomal RNA of Escherichia coli. I. The effect of kasugamycin on initiation of protein synthesis.
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4
Isolation of kasugamycin resistant mutants in the 16 S ribosomal RNA of Escherichia coli.大肠杆菌16S核糖体RNA中抗春日霉素突变体的分离。
J Mol Biol. 1999 Oct 15;293(1):1-8. doi: 10.1006/jmbi.1999.3160.
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Calorimetric measurements of the destabilisation of a ribosomal RNA hairpin by dimethylation of two adjacent adenosines.通过两个相邻腺苷的二甲基化对核糖体RNA发夹结构去稳定化的量热法测量。
Nucleic Acids Res. 1983 Jan 11;11(1):203-10. doi: 10.1093/nar/11.1.203.
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Increased translational fidelity caused by the antibiotic kasugamycin and ribosomal ambiguity in mutants harbouring the ksgA gene.抗生素春日霉素引起的翻译保真度增加以及携带ksgA基因的突变体中的核糖体歧义性。
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Eur J Biochem. 1981 Jan;113(3):587-93. doi: 10.1111/j.1432-1033.1981.tb05103.x.

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Sequence and secondary structure of the colicin fragment of Bacillus stearothermophilus 16S ribosomal RNA.嗜热脂肪芽孢杆菌16S核糖体RNA的大肠杆菌素片段的序列和二级结构
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Studies on the function of two adjacent N6,N6-dimethyladenosines near the 3' end of 16S ribosomal RNA of Escherichia coli. IV. The effect of the methylgroups on ribosomal subunit interaction.大肠杆菌16S核糖体RNA 3'端附近两个相邻的N6,N6-二甲基腺苷功能的研究。IV. 甲基对核糖体亚基相互作用的影响。
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