Kremer R, Guillemant S
Clin Chim Acta. 1978 Jun;86(2):187-94. doi: 10.1016/0009-8981(78)90132-8.
A sensitive and specific competitive protein binding assay for 24,25-dihydroxyvitamin D was developed using rat kidney cytosol as the binding protein. It also included a chromatographic step on a Sephadex LH 20 column to separate the metabolite specifically. The average value for 24, 25-dihydroxyvitamin D in serum in 19 male and female healthy subjects aged from 20 to 40 was found to be 8.24 +/- 0.34 ng/ml (1.98 +/- 0.08 . 10(-8) mol/l) mean +/- S.D. In hepatic insufficiencies the levels are higher (14.15 +/- 3.59 ng/ml) than in normal subjects. A comparison between 25-hydroxyvitamin D and 24,25-dihydroxyvitamin D levels was made.
利用大鼠肾细胞溶质作为结合蛋白，开发了一种灵敏且特异的24,25-二羟基维生素D竞争性蛋白结合测定法。该方法还包括在Sephadex LH 20柱上进行色谱分离步骤，以特异性分离代谢物。在19名年龄在20至40岁的健康男性和女性受试者中，血清中24,25-二羟基维生素D的平均值为8.24±0.34 ng/ml（1.98±0.08.10(-8) mol/l），均值±标准差。在肝功能不全患者中，该水平（14.15±3.59 ng/ml）高于正常受试者。对25-羟基维生素D和24,25-二羟基维生素D水平进行了比较。
