Lund B, Lund B, Sørensen O H
Acta Endocrinol (Copenh). 1979 Jun;91(2):338-50. doi: 10.1530/acta.0.0910338.
A sensitive assay is described for measurement of circulating 1,25-dihydroxyvitamin D (1,25-(OH)2D) using rachitic chick intestinal cytosol binding protein. The metabolite was extracted from serum by diethyl ether and chromotographed on a Sephadex LH-20 column followed by high-pressure liquid chromatography. The concentration of 1,25-(OH)2D was then measured by a competitive protein-binding assay. The binding capacity of the protein was unchanged for 4 months. The inter- and intra-assay variations were both 11%. The standard curve was useful at serum concentrations ranging from 14 to 395 pmol/l (6 to 165 pg/ml). The normal mean concentration was 79.6 +/- 36.8 pmol/l (33.1 +/- 15.3 pg/ml). The serum 1,25-(OH)2D was studied in elderly subjects following administration of 1 alpha-hydroxycholecalciferol (1 alpha-OHD3) at different doses. Large variations occurred indicating individual rates of metabolism of 1,25-(OH)2D.
描述了一种使用佝偻病雏鸡肠道胞质溶胶结合蛋白来测量循环中1,25 - 二羟基维生素D（1,25-(OH)₂D）的灵敏测定法。代谢产物用乙醚从血清中提取，然后在Sephadex LH - 20柱上进行色谱分离，接着进行高压液相色谱分析。然后通过竞争性蛋白结合测定法测量1,25-(OH)₂D的浓度。该蛋白的结合能力在4个月内未发生变化。测定间和测定内的变异均为11%。标准曲线在血清浓度为14至395 pmol/l（6至165 pg/ml）范围内有效。正常平均浓度为79.6±36.8 pmol/l（33.1±15.3 pg/ml）。在老年受试者中，研究了给予不同剂量的1α-羟基胆钙化醇（1α-OHD₃）后血清1,25-(OH)₂D的情况。结果显示存在较大差异，表明1,25-(OH)₂D的个体代谢率不同。
