Feitelson J S, Hopwood D A
Mol Gen Genet. 1983;190(3):394-8. doi: 10.1007/BF00331065.
The red pigmented antibiotic of Streptomyces coelicolor A3(2) is chemically very similar to the Serratia marcescens pigment, prodigiosin. We have demonstrated by co-synthesis experiments between non-producing mutants of both species that their biosynthetic pathways are similar, and have discovered identities between specific mutants of each organism. Molecular cloning techniques have been employed in order to isolate Streptomyces chromosomal DNA segments which "complement" a mutant defective in the penultimate step of the red biosynthetic pathway: an O-methyltransferase enzyme. In one case, the lesion appears to be repaired by integrative recombination into the chromosome; another case may represent expression from the autonomously replicating recombinant plasmid.
天蓝色链霉菌A3(2)产生的红色色素抗生素在化学结构上与粘质沙雷氏菌色素灵菌红素非常相似。我们通过两种菌的非产生突变体之间的共合成实验证明，它们的生物合成途径相似，并且发现了每种生物体特定突变体之间的一致性。为了分离出能“互补”红色生物合成途径倒数第二步缺陷突变体（一种O-甲基转移酶）的链霉菌染色体DNA片段，我们采用了分子克隆技术。在一个案例中，损伤似乎通过整合重组到染色体中得以修复；另一个案例可能代表自主复制重组质粒的表达。
