Raĭnov A, Chobanova D
Eksp Med Morfol. 1978;17(2):85-91.
The authors examined the action of irradiation with ruby lazer at lambda=6,948 A and level of energy 0.13 j/sm2. Liver of rats was irradiated through the surrounding tissues in five successive impulses. The skin in the region of action was stained with methylene blue. Changes in the incorporation of the label in the free amino-acids, in total cholesterol of liver and ribosomal protein as well as the proteins in the supernatant over ribosomes were examined. Glycine (14C) was identified by chromatographic was and autoradiographycaly. The relative radioactivity was determined by a scintillation radiometer. The data, estimated statistically, on the studies showed that 48 hours after lazer irradiation the incorporation of glycine was elevated over 12%. There were no apparent changes macroscopicaly and in the usual histologic preparations during the same period of time. Electronmicroscopic investigation revealed a certain alteration of the membrane structures, vacuolization at sites, but preserved ribosomal structures, accumulation of glycogen. The established changes were described as a manifestation of nonthermic effects of the type "striking wave" with successive increased course of reparative processes.
作者研究了波长为6948埃、能量水平为0.13焦/平方厘米的红宝石激光照射的作用。通过周围组织对大鼠肝脏进行连续五次脉冲照射。用亚甲蓝对作用区域的皮肤进行染色。检测了标记物在游离氨基酸、肝脏总胆固醇和核糖体蛋白以及核糖体上清液中的蛋白质掺入情况的变化。通过色谱法和放射自显影法鉴定甘氨酸（14C）。通过闪烁辐射计测定相对放射性。对研究数据进行统计学估计表明，激光照射48小时后，甘氨酸的掺入量提高了12%以上。在同一时间段内，宏观上和常规组织学标本中均未出现明显变化。电子显微镜检查显示膜结构有一定改变，局部有空泡化，但核糖体结构保存，糖原积累。所确定的变化被描述为“冲击波”类型的非热效应的表现，修复过程呈连续增加的过程。
