Clark D A, McCulloch P B, Liao S K, Dent P B, Fuks A
J Natl Cancer Inst. 1984 Mar;72(3):505-12.
The HCT-8R clone of the HCT-8 human colon tumor line, which expresses increased quantities of carcinoembryonic antigen (CEA) on its surface, was discovered to have an enhanced susceptibility to lysis by natural killer (NK) cells in human peripheral blood. This increase in susceptibility to lysis by peripheral blood mononuclear cells was not explained by stimulation of interferon release by HCT-8R cells but rather was found to be attributable to an increased susceptibility of HCT-8R cells to lysis by those NK cells that bind to sheep erythrocytes (E-RFC). Cold target competition experiments and single-cell assay for cytotoxic cells suggested that the presence of surface CEA did not increase lysis of HCT-8R by facilitating "recognition" by E-RFC-type cytotoxic cells but by rendering HCT-8R cells more susceptible to the lytic mechanism of NK cells. The magnitude of expression of surface CEA by a variety of human carcinoma cell lines with a few exceptions and subclones of HCT-8 also correlated with increased susceptibility to lysis by blood mononuclear cells. The possible clinical significance of these findings was discussed.
在人结肠肿瘤系HCT - 8的HCT - 8R克隆中，发现其表面表达大量癌胚抗原（CEA），对人外周血中的自然杀伤（NK）细胞裂解具有增强的敏感性。外周血单个核细胞对其裂解敏感性的增加并非由HCT - 8R细胞刺激干扰素释放所致，而是发现HCT - 8R细胞对那些与绵羊红细胞结合的NK细胞（E - RFC）的裂解敏感性增加。冷靶竞争实验和细胞毒性细胞单细胞测定表明，表面CEA的存在并非通过促进E - RFC型细胞毒性细胞的“识别”来增加对HCT - 8R的裂解，而是使HCT - 8R细胞对NK细胞的裂解机制更敏感。除少数例外，多种人癌细胞系以及HCT - 8的亚克隆表面CEA的表达量也与对血液单个核细胞裂解的敏感性增加相关。讨论了这些发现可能的临床意义。
