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一个可弥补支链氨基酸转氨酶缺陷的基因在人类12号染色体短臂上的定位。

Localization of a gene which complements branched-chain amino acid transaminase deficiency to the short arm of human chromosome 12.

作者信息

Jones C, Moore E E

出版信息

Hum Genet. 1984;66(2-3):206-11. doi: 10.1007/BF00286602.

DOI:10.1007/BF00286602
PMID:6585344
Abstract

A human X Chinese hamster somatic cell hybrid (J1) containing only one human chromosome (number 11) does not express branched-chain amino acid transaminase and, therefore, cannot grow if the branched-alpha-keto acids are substituted for the branched-chain amino acids in the growth medium. J1 cells, which are glycine auxotrophs (Gly-A), were fused with normal human lymphocytes and the resulting hybrids selectively isolated in glycine-free medium. A total of 16 primary and 46 secondary clones were analyzed for isozymic and nutritional markers. Cytogenetic analysis with chromosome banding was also performed on selected hybrid clones. The results provide evidence that branched-chain amino acid transaminase is syntenic with lactic dehydrogenase B and is located on the short arm of human chromosome 12.

摘要

一种仅含有一条人类染色体（11号染色体）的人X中国仓鼠体细胞杂种（J1）不表达支链氨基酸转氨酶，因此，如果在生长培养基中用支链α-酮酸替代支链氨基酸，它就无法生长。J1细胞是甘氨酸营养缺陷型（Gly-A），与正常人淋巴细胞融合，并在无甘氨酸培养基中选择性分离得到杂种细胞。对总共16个原代克隆和46个二代克隆进行了同工酶和营养标记分析。还对选定的杂种克隆进行了染色体显带的细胞遗传学分析。结果提供了证据，表明支链氨基酸转氨酶与乳酸脱氢酶B是同线的，并且位于人类12号染色体的短臂上。

相似文献

1
Localization of a gene which complements branched-chain amino acid transaminase deficiency to the short arm of human chromosome 12.一个可弥补支链氨基酸转氨酶缺陷的基因在人类12号染色体短臂上的定位。
Hum Genet. 1984;66(2-3):206-11. doi: 10.1007/BF00286602.
2
Branched-chain aminotransferase deficiency in Chinese hamster cells complemented by two independent genes on human chromosomes 12 and 19.
Somatic Cell Genet. 1980 Sep;6(5):641-52. doi: 10.1007/BF01538643.
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本文引用的文献

1
CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM.哺乳动物细胞在化学成分明确的合成培养基中的克隆生长
Proc Natl Acad Sci U S A. 1965 Feb;53(2):288-93. doi: 10.1073/pnas.53.2.288.
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Giemsa-11 technique. Applications in the chromosomal characterization of hematologic specimens.吉姆萨-11技术。在血液学标本染色体特征分析中的应用。
Hum Genet. 1982;61(2):141-4. doi: 10.1007/BF00274204.
3
Chromosomal assignment of the gene for folylpolyglutamate synthetase to human chromosome 9.叶酰聚谷氨酸合成酶基因在人类9号染色体上的定位
Cytogenet Cell Genet. 1980;28(3):181-94. doi: 10.1159/000131529.
4
Branched-chain aminotransferase deficiency in Chinese hamster cells complemented by two independent genes on human chromosomes 12 and 19.
Somatic Cell Genet. 1980 Sep;6(5):641-52. doi: 10.1007/BF01538643.
5
Transaminase of branched chain amino acids. I. Branched chain amino acids-alpha-ketoglutarate transaminase.支链氨基酸转氨酶。I. 支链氨基酸-α-酮戊二酸转氨酶。
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Genetics of somatic mammalian cells: linkage studies with human-Chinese hamster cell hybrids.体细胞哺乳动物细胞遗传学：与人类-中国仓鼠细胞杂交体的连锁研究。
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7
Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants.哺乳动物体细胞遗传学。X. 甘氨酸需求型突变体的互补分析。
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1284-91. doi: 10.1073/pnas.64.4.1284.
8
Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.哺乳动物体细胞遗传学，VII. 中国仓鼠细胞中营养缺陷型突变体的诱导与分离
Proc Natl Acad Sci U S A. 1968 Aug;60(4):1275-81. doi: 10.1073/pnas.60.4.1275.
9
Assignment of the genes for malate oxidoreductase decarboxylating to chromosome 6 and peptidase B and lactate dehydrogenase B to chromosome 12 in man.人类中苹果酸氧化还原酶脱羧基因定位于6号染色体，肽酶B和乳酸脱氢酶B基因定位于12号染色体。
Am J Hum Genet. 1973 Mar;25(2):200-7.
10
Enzyme electrophoresis on cellulose acetate gel. II. Zymogram patterns in man-Chinese hamster somatic cell hybrids.醋酸纤维素凝胶上的酶电泳。II. 人 - 中国仓鼠体细胞杂种中的酶谱模式。
Humangenetik. 1974;25(3):189-201. doi: 10.1007/BF00281426.