Mascarello J T, Soderberg K, Scheffler I E
Cytogenet Cell Genet. 1980;28(1-2):121-35. doi: 10.1159/000131520.
A Chinese hamster cell mutant has been described with little or no activity of succinate dehydrogenase (SODERBERG et al., 1977). We described here the selection and characterization of human-hamster hybrids obtained from the fusion of these mutant cells and human lymphoblasts or HT1080 fibrosarcoma cells. The presence of human chromosome 1, identified by cytogenetic techniques and isozyme analysis, is correlated with the restoration of succinate dehydrogenase activity in the hybrids, and segregants are described in which the loss of all or part of human chromosome 1 has also led to a loss of this activity. We present in one of the two structural genes for the 70,000 and 30,000 dalton peptides, respectively, which constitute succinate dehydrogenase. One of these two genes is therefore mapped on human chromosome 1.
已描述了一种中国仓鼠细胞突变体，其琥珀酸脱氢酶活性很低或没有活性（索德伯格等人，1977年）。我们在此描述了从这些突变细胞与人类淋巴细胞或HT1080纤维肉瘤细胞融合获得的人-仓鼠杂种的筛选和特性。通过细胞遗传学技术和同工酶分析鉴定出的人类1号染色体的存在，与杂种中琥珀酸脱氢酶活性的恢复相关，并且描述了分离体，其中全部或部分人类1号染色体的丢失也导致了这种活性的丧失。我们分别在构成琥珀酸脱氢酶的70,000和30,000道尔顿肽的两个结构基因之一中进行了定位。因此，这两个基因之一被定位在人类1号染色体上。
