Thimmappaya B, Weinberger C, Schneider R J, Shenk T
Cell. 1982 Dec;31(3 Pt 2):543-51. doi: 10.1016/0092-8674(82)90310-5.
Two adenovirus type 5 mutants were constructed to probe the function of the virus-encoded RNA polymerase III transcripts (VA RNAs). Each mutant fails to synthesize one of the two VA RNA species. The variant that does not produce the minor VAII species grows normally. The mutant that cannot synthesize the major VAI species grows more poorly than its parent. Analysis of the mutant's growth defect indicates that the adenovirus VAI RNA is required for efficient translation of viral mRNAs at late times after infection.
构建了两种5型腺病毒突变体，以探究病毒编码的RNA聚合酶III转录本（VA RNA）的功能。每个突变体都无法合成两种VA RNA中的一种。不产生次要VAII种类的变体正常生长。无法合成主要VAI种类的突变体生长情况比其亲本差。对该突变体生长缺陷的分析表明，腺病毒VAI RNA在感染后的晚期对于病毒mRNA的有效翻译是必需的。
