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SW-1271人肺癌细胞N-ras癌基因的激活机制。

Mechanism of activation of an N-ras oncogene of SW-1271 human lung carcinoma cells.

作者信息

Yuasa Y, Gol R A, Chang A, Chiu I M, Reddy E P, Tronick S R, Aaronson S A

出版信息

Proc Natl Acad Sci U S A. 1984 Jun;81(12):3670-4. doi: 10.1073/pnas.81.12.3670.

DOI:10.1073/pnas.81.12.3670
PMID:6587382
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC345280/
Abstract

An N-ras-related transforming gene was detected in the human lung carcinoma cell line SW-1271 and molecularly cloned. The lesion responsible for its acquisition of transforming activity was localized to a single nucleotide transition from A to G in codon 61 of the predicted protein. This lesion in the second exon results in the substitution of arginine for glutamine at this position. These findings, together with previous studies, indicate that the activation of ras oncogenes in human tumors is most commonly due to point mutations at one of two major "hot spots" in the ras coding sequence.

摘要

在人肺癌细胞系SW - 1271中检测到一个与N - ras相关的转化基因并进行了分子克隆。导致其获得转化活性的损伤定位在预测蛋白第61密码子处从A到G的单个核苷酸转变。第二外显子中的这一损伤导致该位置的谷氨酰胺被精氨酸取代。这些发现与先前的研究一起表明，人类肿瘤中ras癌基因的激活最常见的原因是ras编码序列中两个主要“热点”之一的点突变。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1860/345280/4f08d1153311/pnas00613-0072-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1860/345280/681fd9627b77/pnas00613-0071-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1860/345280/4f08d1153311/pnas00613-0072-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1860/345280/681fd9627b77/pnas00613-0071-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1860/345280/4f08d1153311/pnas00613-0072-a.jpg

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本文引用的文献

1
Trans-complementable copy-number mutants of plasmid ColE1.质粒ColE1的反式可互补拷贝数突变体
Nature. 1980 Jan 10;283(5743):216-8. doi: 10.1038/283216a0.
2
Activation of the T24 bladder carcinoma transforming gene is linked to a single amino acid change.T24膀胱癌转化基因的激活与单个氨基酸变化有关。
Nature. 1982 Dec 23;300(5894):762-5. doi: 10.1038/300762a0.
3
Oncogenes in solid human tumours.人类实体瘤中的癌基因。
Gene Regul Syst Bio. 2010 Mar 24;4:19-34. doi: 10.4137/grsb.s4509.
4
Role of transcription-coupled DNA repair in susceptibility to environmental carcinogenesis.转录偶联DNA修复在环境致癌易感性中的作用。
Environ Health Perspect. 1996 May;104 Suppl 3(Suppl 3):547-51. doi: 10.1289/ehp.96104s3547.
5
Three different mutations in codon 61 of the human N-ras gene detected by synthetic oligonucleotide hybridization.通过合成寡核苷酸杂交检测到人类N-ras基因第61密码子的三种不同突变。
Nucleic Acids Res. 1984 Dec 11;12(23):9155-63. doi: 10.1093/nar/12.23.9155.
6
A position 12-activated H-ras oncogene in all HS578T mammary carcinosarcoma cells but not normal mammary cells of the same patient.在所有HS578T乳腺肉瘤癌细胞中存在12位激活的H-ras癌基因，但同一患者的正常乳腺细胞中不存在。
Proc Natl Acad Sci U S A. 1984 Sep;81(17):5384-8. doi: 10.1073/pnas.81.17.5384.
7
Effects of fructose on human fibroblast metabolism: the application of DNA measurements as a basis for interpretation.果糖对人成纤维细胞代谢的影响：将DNA测量作为解释依据的应用。
In Vitro Cell Dev Biol. 1985 Jun;21(6):328-32. doi: 10.1007/BF02691580.
8
Effects of two major activating lesions on the structure and conformation of human ras oncogene products.两种主要激活性损伤对人ras癌基因产物结构和构象的影响。
Proc Natl Acad Sci U S A. 1985 Jan;82(1):38-42. doi: 10.1073/pnas.82.1.38.
9
Loss of the normal N-ras allele in a mouse thymic lymphoma induced by a chemical carcinogen.化学致癌物诱发的小鼠胸腺淋巴瘤中正常N-ras等位基因的缺失。
Proc Natl Acad Sci U S A. 1985 Dec;82(23):7810-4. doi: 10.1073/pnas.82.23.7810.
10
Gangliosides as bimodal regulators of cell growth.神经节苷脂作为细胞生长的双功能调节剂。
Proc Natl Acad Sci U S A. 1987 Jan;84(1):141-5. doi: 10.1073/pnas.84.1.141.
Nature. 1982 Dec 9;300(5892):539-42. doi: 10.1038/300539a0.
4
A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene.一个点突变导致了T24人膀胱癌癌基因获得转化特性。
Nature. 1982 Nov 11;300(5888):149-52. doi: 10.1038/300149a0.
5
Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells.从T24膀胱癌细胞中分离出一种人类转化基因并进行初步鉴定。
Nature. 1982 Apr 1;296(5856):404-9. doi: 10.1038/296404a0.
6
Oncogenes in human tumor cell lines: molecular cloning of a transforming gene from human bladder carcinoma cells.人类肿瘤细胞系中的癌基因：从人膀胱癌细胞中克隆一个转化基因
Proc Natl Acad Sci U S A. 1982 May;79(9):2845-9. doi: 10.1073/pnas.79.9.2845.
7
Transforming activity of human tumor DNAs.人类肿瘤DNA的转化活性。
Proc Natl Acad Sci U S A. 1981 Feb;78(2):1181-4. doi: 10.1073/pnas.78.2.1181.
8
Acquisition of transforming properties by alternative point mutations within c-bas/has human proto-oncogene.c-bas/has人类原癌基因内的替代点突变导致转化特性的获得。
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9
The HL-60 transforming sequence: a ras oncogene coexisting with altered myc genes in hematopoietic tumors.HL-60转化序列：一种与造血肿瘤中改变的myc基因共存的ras癌基因。
Cell. 1983 Jul;33(3):749-57. doi: 10.1016/0092-8674(83)90017-x.
10
Structure and activation of the human N-ras gene.人类N-ras基因的结构与激活
Cell. 1983 Sep;34(2):581-6. doi: 10.1016/0092-8674(83)90390-2.