Camosci D A, Tinanoff N
J Dent Res. 1984 Sep;63(9):1121-5. doi: 10.1177/00220345840630090501.
The growth of bacteria on stainless steel wires was used as a model to investigate which properties of SnF2 produced anti-bacterial effects against S. mutans. Wire-adherent bacteria were exposed for one min, twice a day, to various fluoride or control compounds having similar ions, pH, valence, or atomic weights. After two days, the thickness of adherent bacteria was scored visually, and the decrease in pH of the growth medium was determined. Bacteria from each wire were then dried, weighed, and analyzed for metal content. Electron microscopy and electron microprobe were used to identify the location of heavy metal deposits in the bacteria. Only SnF2 dramatically altered S. mutans growth and metabolism, and this anti-bacterial effect was associated with a large uptake of tin into the bacterial cells. The fluoride salts of sodium, lead, zinc, and copper had little influence on S. mutans growth in this test system. The pH of the various fluoride salts or controls generally had no effect on the activity of the test compounds, except for the noted inactivation of SnF2 at elevated pH's. Since SnF4 did not alter the growth or metabolism of S. mutans, a unique property of SnF2--possibly the reactivity in an aqueous environment--may be responsible for its anti-bacterial properties.
以不锈钢丝上细菌的生长作为模型，来研究氟化亚锡的哪些特性对变形链球菌产生抗菌作用。将附着在钢丝上的细菌每天两次、每次暴露于含有相似离子、pH值、化合价或原子量的各种氟化物或对照化合物中1分钟。两天后，通过肉眼观察对附着细菌的厚度进行评分，并测定生长培养基pH值的降低情况。然后将每根钢丝上的细菌干燥、称重并分析金属含量。使用电子显微镜和电子微探针来确定细菌中重金属沉积物的位置。只有氟化亚锡显著改变了变形链球菌的生长和代谢，并且这种抗菌作用与大量锡摄入细菌细胞有关。在该测试系统中，钠、铅、锌和铜的氟化物盐对变形链球菌的生长几乎没有影响。除了在较高pH值下氟化亚锡明显失活外，各种氟化物盐或对照的pH值通常对测试化合物的活性没有影响。由于四氟化锡不会改变变形链球菌的生长或代谢，氟化亚锡的一种独特特性——可能是在水环境中的反应性——可能是其抗菌特性的原因。
