人胰蛋白酶、糜蛋白酶B和弹性蛋白酶等丝氨酸蛋白酶基因的染色体定位。

Chromosomal assignments of human genes for serine proteases trypsin, chymotrypsin B, and elastase.

作者信息

Honey N K, Sakaguchi A Y, Quinto C, MacDonald R J, Bell G I, Craik C, Rutter W J, Naylor S L

出版信息

Somat Cell Mol Genet. 1984 Jul;10(4):369-76. doi: 10.1007/BF01535632.

Abstract

The genes for the serine proteases trypsin, chymotrypsin B, and elastase were chromosomally assigned in man using cDNA probes that have been isolated from a rat pancreatic cDNA library. DNA from human X rodent somatic cell hybrids was cleaved with BamHI or EcoRI and analyzed by Southern filter hybridization methods for the segregation of the genes for trypsin-1 (TRY1), chymotrypsin B (CTRB), and elastase-1 (ELA1). TRY1 was assigned to human chromosome 7q22----qter, CTRB to chromosome 16, and ELA1 to chromosome 12. Although the three genes are members of the same gene family, they are dispersed over different chromosomes.

摘要

利用从大鼠胰腺cDNA文库中分离得到的cDNA探针，将人丝氨酸蛋白酶胰蛋白酶、糜蛋白酶B和弹性蛋白酶的基因进行了染色体定位。用人-啮齿动物体细胞杂种的DNA用BamHI或EcoRI酶切，并用Southern印迹杂交法分析胰蛋白酶-1（TRY1）、糜蛋白酶B（CTRB）和弹性蛋白酶-1（ELA1）基因的分离情况。TRY1定位于人染色体7q22----qter，CTRB定位于染色体16，ELA1定位于染色体12。尽管这三个基因是同一基因家族的成员，但它们分散在不同的染色体上。