小鼠中胰蛋白酶、胰凝乳蛋白酶B和弹性蛋白酶基因的染色体定位。

Chromosomal assignments of genes for trypsin, chymotrypsin B, and elastase in mouse.

作者信息

Honey N K, Sakaguchi A Y, Lalley P A, Quinto C, MacDonald R J, Craik C, Bell G I, Rutter W J, Naylor S L

出版信息

Somat Cell Mol Genet. 1984 Jul;10(4):377-83. doi: 10.1007/BF01535633.

Abstract

The mouse genes for the serine proteases trypsin (Try-1), chymotrypsin B (Ctrb), and elastase (Ela-1) were chromosomally assigned using Southern blot hybridization of mouse X Chinese hamster cell hybrid DNA. cDNA probes for the three genes were hybridized to cell hybrid DNA cleaved with BamHI or HindIII and the segregation of Try-1, Ctrb, and Ela-1 was correlated with the segregation of mouse chromosomes. Try-1 is located on chromosome 6, Ctrb is on chromosome 8, and Ela-1 is on chromosome 15. The three genes fall into three syntenic groups that are conserved in the mouse and human genomes.

摘要

利用小鼠X中国仓鼠细胞杂交DNA的Southern印迹杂交技术，对丝氨酸蛋白酶胰蛋白酶（Try-1）、胰凝乳蛋白酶B（Ctrb）和弹性蛋白酶（Ela-1）的小鼠基因进行了染色体定位。将这三个基因的cDNA探针与用BamHI或HindIII切割的细胞杂交DNA进行杂交，并将Try-1、Ctrb和Ela-1的分离与小鼠染色体的分离相关联。Try-1位于6号染色体上，Ctrb位于8号染色体上，Ela-1位于15号染色体上。这三个基因属于三个同线群，在小鼠和人类基因组中是保守的。