两种主要梯牧草花粉过敏原对四种禾本科植物全花粉提取物的免疫学部分同一性及体外抑制作用

Immunological partial identity and in vitro inhibitory effect of two major timothy pollen allergens to whole pollen extract of four grasses.

作者信息

Løwenstein H

出版信息

Int Arch Allergy Appl Immunol. 1978;57(4):379-83. doi: 10.1159/000232128.

Abstract

Crossed immunoelectrophoresis in various modifications has directly revealed the immunological partial identity between isolated major allergens of timothy pollen (antigens 19 and 25) and the corresponding pollen allergens of false oat, orchard and rye grass, using rabbit antibodies raised against all four grasses. The inhibitory effects of antigens 19 and 25 relative to those of whole pollen extracts have been determined by means of RAST, inhibiting serum pools from selected grass-pollen-allergic patients and using insolubilized whole pollen extracts from the four grasses as allergosorbents. The inhibitory effects of antigens 19 and 25 were determined to be 97, 91, 92 and 73% of those of whole extracts of timothy, false oat, orchard and rye grass, respectively.

摘要

采用针对所有四种草产生的兔抗体，通过各种改良的交叉免疫电泳法，直接揭示了梯牧草花粉分离出的主要变应原（抗原19和25）与假燕麦、果园草和黑麦草相应花粉变应原之间的免疫部分同一性。通过放射变应原吸附试验（RAST），使用从选定的对草花粉过敏患者中提取的抑制性血清库，并以四种草的不溶性全花粉提取物作为变应原吸附剂，确定了抗原19和25相对于全花粉提取物的抑制作用。抗原19和25的抑制作用分别被确定为梯牧草、假燕麦、果园草和黑麦草全提取物抑制作用的97%、91%、92%和73%。