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人类主要组织相容性复合体内I类DNA序列的图谱绘制。

Mapping of class I DNA sequences within the human major histocompatibility complex.

作者信息

Orr H T, DeMars R

出版信息

Immunogenetics. 1983;18(5):489-502. doi: 10.1007/BF00364390.

DOI:10.1007/BF00364390
PMID:6315574
Abstract

Mutants that had lost expression of alleles of one or more HLA loci were isolated with immunoselection after gamma-irradiation of a human lymphoblastoid cell line LCL 721. DNAs from the mutants were digested with restriction endonucleases and analyzed by Southern blotting using probes for class I HLA genes. Eight polymorphic cut sites for HindIII and PvuII were discovered in class I-associated sequences of LCL 721. Losses of specific fragments generated by restriction enzymes could be associated with losses of specific antigenic expressions and it was possible in this way to assign HLA-A1, HLA-A2, and HLA-B8 to specific DNA fragments. Patterns of gamma-ray-induced segregations of DNA fragments permitted rough linkage alignment of about 30% of the fragments generated by PvuII. The resultant map showed that there are class I HLA genes on the telomeric side of the HLA-A locus. Restriction enzyme site polymorphisms were also examined in a panel of DNAs isolated from peripheral blood lymphocytes (PBLs) of HLA-typed individuals. This panel of PBL DNA complemented the analysis using the HLA deletion mutants.

摘要

在对人淋巴母细胞系LCL 721进行γ射线照射后，通过免疫选择分离出一个或多个HLA位点等位基因表达缺失的突变体。用限制性内切酶消化突变体的DNA，并用I类HLA基因探针通过Southern印迹法进行分析。在LCL 721的I类相关序列中发现了HindIII和PvuII的八个多态性切割位点。限制性酶产生的特定片段的缺失可能与特定抗原表达的缺失有关，通过这种方式可以将HLA - A1、HLA - A2和HLA - B8分配到特定的DNA片段。γ射线诱导的DNA片段分离模式允许对PvuII产生的约30%的片段进行粗略的连锁排列。所得图谱显示，在HLA - A位点的端粒侧存在I类HLA基因。还在从HLA分型个体的外周血淋巴细胞（PBL）中分离的一组DNA中检查了限制性酶切位点多态性。这组PBL DNA补充了使用HLA缺失突变体的分析。

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Mapping of class I DNA sequences within the human major histocompatibility complex.人类主要组织相容性复合体内I类DNA序列的图谱绘制。
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2
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引用本文的文献

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Proc Natl Acad Sci U S A. 1984 Aug;81(16):5175-8. doi: 10.1073/pnas.81.16.5175.
2
Mutations that impair a posttranscriptional step in expression of HLA-A and -B antigens.损害HLA - A和 - B抗原表达转录后步骤的突变。
Proc Natl Acad Sci U S A. 1985 Dec;82(23):8183-7. doi: 10.1073/pnas.82.23.8183.
3
Unusual RNA splicing generates a secreted form of HLA-A2 in a mutagenized B lymphoblastoid cell line.异常的RNA剪接在诱变的B淋巴母细胞系中产生一种分泌形式的HLA - A2。

本文引用的文献

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Nature. 1982 Apr 1;296(5856):454-6. doi: 10.1038/296454a0.
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Transfer of cloned human class I major histocompatibility complex genes into HLA mutant human lymphoblastoid cells.克隆的人类I类主要组织相容性复合体基因向HLA突变型人类淋巴母细胞样细胞的转移。
Mol Cell Biol. 1986 Apr;6(4):1074-87. doi: 10.1128/mcb.6.4.1074-1087.1986.
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Construction of a map of the short arm of human chromosome 6.人类6号染色体短臂图谱的构建。
Proc Natl Acad Sci U S A. 1986 Jun;83(11):3909-13. doi: 10.1073/pnas.83.11.3909.
10
The HLA-AW24 gene: sequence, surroundings and comparison with the HLA-A2 and HLA-A3 genes.HLA - AW24基因：序列、周边情况以及与HLA - A2和HLA - A3基因的比较
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Science. 1982 Feb 5;215(4533):677-9. doi: 10.1126/science.7058331.
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Hum Immunol. 1981 Dec;3(4):277-99. doi: 10.1016/0198-8859(81)90065-3.
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Identification of human genomic clones coding the major histocompatibility antigens HLA-a2 and HLA-B7 by DNA-mediated gene transfer.通过DNA介导的基因转移鉴定编码主要组织相容性抗原HLA-a2和HLA-B7的人类基因组克隆。
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Structure and expression of a mouse major histocompatibility antigen gene, H-2Ld.小鼠主要组织相容性抗原基因H-2Ld的结构与表达
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