影响衣原体A（沙眼包涵体结膜炎病原体）分离和生长过程中 McCoy 传代细胞敏感性的因素
Factors affecting the sensitivity of replicating McCoy cells in the isolation and growth of chlamydia A (TRIC agents).
作者信息
Johnson F W, Hobson D
出版信息
J Hyg (Lond). 1976 Jun;76(3):441-51. doi: 10.1017/s0022172400055376.
Abstract
Normal non-irradiated McCoy cell cultures provide a sensitive and reproducible method for the isolation of oculo-genital strains of chlamydia A directly from human secretions and for laboratory studies with these agents. Since September 1973, chlamydia have been isolated from 175 of 562 women (32-1%) attending venereal disease clinics. Freshly isolated and low passage strains have been used to determine the importance of centrifugation, constitution and pH of the tissue culture medium, and the temperature of incubation in controlling the efficiency of plating in the method.
摘要
正常未受辐照的 McCoy 细胞培养物为直接从人体分泌物中分离沙眼衣原体 A 的眼-生殖器菌株以及对这些病原体进行实验室研究提供了一种灵敏且可重复的方法。自 1973 年 9 月以来，在 562 名前往性病诊所就诊的女性中，有 175 人（32.1%）分离出了衣原体。新分离出的低传代菌株已被用于确定离心、组织培养基的组成和 pH 值以及培养温度对该方法中接种效率控制的重要性。
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