促癌佛波酯对裸鼠卵母细胞减数分裂成熟的抑制作用及其被类视黄醇的逆转作用。

Inhibition of denuded mouse oocyte meiotic maturation by tumor-promoting phorbol esters and its reversal by retinoids.

作者信息

Urner F, Schorderet-Slatkine S

出版信息

Exp Cell Res. 1984 Oct;154(2):600-5. doi: 10.1016/0014-4827(84)90184-8.

Abstract

Two tumor-promoting phorbol esters, phorbol 12,13-dibutyrate (PDBu) and phorbol 12-myristate 13-acetate (PMA), when added to the culture medium of denuded mouse oocytes prevent their spontaneous meiotic maturation, whereas phorbol 13-acetate, which is inactive as a tumor promoter, does not inhibit this process. Retinoids appear to antagonize this inhibitory action of tumor promoters. However, the inhibitory effect of forskolin on meiotic maturation is not prevented, but is potentiated by retinal. These data indirectly suggest a role for calcium and/or phospholipids in the regulation of meiotic maturation. They also suggest that forskolin and phorbol esters mediate their effects through different pathways.

摘要

两种促肿瘤佛波酯，佛波醇12,13 - 二丁酸酯（PDBu）和佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯（PMA），添加到去除卵丘细胞的小鼠卵母细胞培养基中时，可阻止其自发减数分裂成熟，而作为肿瘤促进剂无活性的佛波醇13 - 乙酸酯则不抑制此过程。类视黄醇似乎可拮抗肿瘤促进剂的这种抑制作用。然而，福司可林对减数分裂成熟的抑制作用并未被阻止，反而被视黄醛增强。这些数据间接表明钙和/或磷脂在减数分裂成熟的调节中起作用。它们还表明福司可林和佛波酯通过不同途径介导其作用。