Mizoguchi A, Takasaki S, Maeda S, Kobata A
J Biol Chem. 1984 Oct 10;259(19):11943-8.
Structural changes in the asparagine-linked sugar chains of plasma membrane glycoproteins during myeloid and monocytoid differentiation were investigated by the use of an in vitro differentiation system for human promyelocytic leukemic cells (HL-60), which can be induced to more mature myeloid cells by exposure to dimethyl sulfoxide and to macrophage-like cells by a phorbol ester. The asparagine-linked sugar chains released from their plasma membranes by hydrazinolysis were separated into a neutral fraction and an acidic fraction composed of over ten components. The content of neutral oligosaccharides, which accounted for 8% of the total asparagine-linked sugar chains in HL-60 cells, increased slightly to 13% in dimethyl sulfoxide-induced cells and markedly to 33% in phorbol ester-induced cells. Structural analyses revealed that the neutral oligosaccharides of HL-60 cells are all of the complex type with a variety of Gal beta 1----4GlcNAc beta 1----units in their outer chain moieties and the following core structure: (sequence; see text) After myeloid and monocytoid differentiation, the total amount of neutral complex-type sugar chains did not change significantly, but newly found high mannose-type sugar chains contributed up to 3% and 24% of the total sugar chains, respectively.
利用人早幼粒细胞白血病细胞（HL - 60）的体外分化系统，研究了髓系和单核细胞系分化过程中质膜糖蛋白中天冬酰胺连接糖链的结构变化。HL - 60细胞可通过暴露于二甲基亚砜诱导分化为更成熟的髓系细胞，通过佛波酯诱导分化为巨噬细胞样细胞。通过肼解从其质膜释放的天冬酰胺连接糖链被分离为中性部分和由十多种成分组成的酸性部分。中性寡糖的含量在HL - 60细胞中占总天冬酰胺连接糖链的8%，在二甲基亚砜诱导的细胞中略有增加至13%，在佛波酯诱导的细胞中显著增加至33%。结构分析表明，HL - 60细胞的中性寡糖均为复合型，其外链部分具有多种Galβ1----4GlcNAcβ1----单元以及以下核心结构：（序列；见原文）在髓系和单核细胞系分化后，中性复合型糖链的总量没有显著变化，但新发现的高甘露糖型糖链分别占总糖链的3%和24%。
