DenBesten P K, Crenshaw M A
Arch Oral Biol. 1984;29(9):675-9. doi: 10.1016/0003-9969(84)90171-7.
Sixty-gramme rats were given either 0, 75, 100 or 150 parts/10(6) fluoride in their drinking water. After five weeks, the fluoride, the phosphorus and the protein contents of the enamel were compared in control and experimental animals at three stages of enamel development. The mineral content was reduced in pigmented enamel from animals given 75 parts/10(6) or more fluoride in their drinking water. The fluoride content was elevated in all stages of fluorosed enamel development. At the lowest fluoride level (75 parts/10(6], a larger proline content was found in the proteins of the maturing, fluorosed enamel but there was no increase in the protein content. In animals given 100 parts/10(6) fluoride in their drinking water, the proline content of the protein was greater in maturing, fluorosed enamel, and the total protein content of the post-secretory enamel (maturing and pigmented) was greater than in the controls. These observations indicate that, with increasing levels of fluoride in drinking water, there was an initial delay in the loss of the amelogenin proteins followed by a decreased removal of total protein from the enamel. These results indicate that fluoride interfered with the normal post-secretory, pre-eruptive development of enamel.
给60克重的大鼠饮用含氟量分别为0、75、100或150 ppm（百万分之一）的水。五周后，在牙釉质发育的三个阶段，对对照组和实验组动物牙釉质中的氟、磷和蛋白质含量进行了比较。饮用含氟量为75 ppm及以上的动物，其着色牙釉质中的矿物质含量降低。在氟斑牙釉质发育的各个阶段，氟含量均升高。在最低氟含量水平（75 ppm）时，成熟的氟斑牙釉质蛋白质中的脯氨酸含量较高，但蛋白质含量并未增加。饮用含氟量为100 ppm的动物，成熟的氟斑牙釉质蛋白质中的脯氨酸含量更高，分泌后牙釉质（成熟和着色）的总蛋白质含量高于对照组。这些观察结果表明，随着饮用水中氟含量的增加，釉原蛋白的损失最初会延迟，随后牙釉质中总蛋白质的去除减少。这些结果表明，氟干扰了牙釉质正常的分泌后、萌出前发育。
