Suzuki S, Kitai H, Tojo R, Seki K, Oba M, Fujiwara T, Iizuka R
Fertil Steril. 1981 Feb;35(2):142-8.
Of follicular oocytes with germinal vesicles, 79% resumed meiosis within 48 hours in modified Ham's F-10 medium for oocyte culture, and 59% of them reached the second-metaphase stage of meiosis. An increase in cortical granules and tubular aggregates and a decrease in the Golgi apparatus were observed during the maturation process. Scanning electron microscopy of the oocyte surface revealed a decrease in number and length of microvilli. Nuclear DNA contents diminished to approximately one-half, but cytoplasmic protein contents measured by cytofluorometry were unchanged after culture in spite of these structural changes. Insemination with washed spermatozoa resulted in fertilization of 5 of 43 zona-intact cultured oocytes. After removal of the zona, 6 of 17 cultured oocytes showed polyspermic penetration, although immature oocytes were not fertilized even after removal of the zona. Cultured granulosa cells secreted progesterone spontaneously, and its amount was correlated with follicular size. Ultrastructurally, mitochondria with laminar cristae, enlarged nuclei, dispersed Golgi apparatus, smooth endoplasmic reticulum, lipid droplets in the ooplasm, and granular cytoplasmic protrusions were observed on the oocyte surface.
在含有生发泡的卵泡卵母细胞中，79%在用于卵母细胞培养的改良哈姆氏F-10培养基中48小时内恢复减数分裂，其中59%达到减数分裂的第二次中期阶段。在成熟过程中观察到皮质颗粒和管状聚集体增加，高尔基体减少。卵母细胞表面的扫描电子显微镜显示微绒毛数量和长度减少。核DNA含量减少至大约一半，但尽管有这些结构变化，通过细胞荧光测定法测量的细胞质蛋白质含量在培养后未发生改变。用洗涤过的精子进行授精，43个 zona完整的培养卵母细胞中有5个受精。去除透明带后，17个培养的卵母细胞中有6个显示多精受精，尽管未成熟卵母细胞即使去除透明带后也未受精。培养的颗粒细胞自发分泌孕酮，其分泌量与卵泡大小相关。在超微结构上，观察到卵母细胞表面有线状嵴的线粒体、增大的细胞核、分散的高尔基体、光滑内质网、卵质中的脂滴以及颗粒状细胞质突起。
