Minami S A, Goodenough U W
J Cell Biol. 1978 Apr;77(1):165-81. doi: 10.1083/jcb.77.1.165.
Within the first hour of zygote maturation, Chlamydomonas reinhardtii cells stop synthesizing certain polypeptides that characterize the vegetative and gametic stages of the life cycle and initiate the synthesis of novel, zygote-specific polypeptides. At least six of these polypeptides are secreted into the medium, and fine-structural studies indicate that they represent components of the cell wall that is synthesized and secreted early in zygote development. We conclude that a new program of protein synthesis, and possibly also gene transcription, is initiated shortly after gametic cells fuse, a program that appears highly suited to cell-differentiation studies.
在合子成熟的第一个小时内，莱茵衣藻细胞停止合成某些表征生命周期营养阶段和配子阶段的多肽，并开始合成新的、合子特异性多肽。这些多肽中至少有六种分泌到培养基中，精细结构研究表明它们代表合子发育早期合成并分泌的细胞壁成分。我们得出结论，在配子细胞融合后不久就启动了一个新的蛋白质合成程序，可能还有基因转录程序，这个程序似乎非常适合细胞分化研究。
