Malveaux F J, Conroy M C, Adkinson N F, Lichtenstein L M
J Clin Invest. 1978 Jul;62(1):176-81. doi: 10.1172/JCI109103.
As reported previously, and confirmed here in 26 donors, the serum IgE level (2.6-5,500 ng/ml) correlates well (rs = 0.95, P less than 0.001) with the in vivo number of IgE molecules/basophil (6,000-600,000). The total number of IgE receptors/basophil was monitored by incubating them with an IgE-rich serum (15 microgram/ml), quantitatively stripping IgE from the cells at pH 3.7, and measuring eluted IgE by a direct radioimmunosorbent test. Saturation of receptors for each donor was achieved with 15 nM IgE (3 microgram/ml). The proportion of receptors occupied in vivo correlated with the serum IgE (rs = 0.84, P less than 0.001) whereas the average association constant of the receptors was independent of serum IgE and ranged from 7.1 X 10(8)/M to 2.8 X 10(10)/M, averaging 7.7 X 10(9)/M. Unexpectedly, the total number of IgE receptors/basophil was closely related to the serum IgE level. (rs = 0.92, P less than 0.001). Thus, either there is genetic association between serum IgE and the number of basophil IgE receptors, or, more likely, the receptor number is modulated by the serum IgE concentration.
如先前报道，并在此对26名供体进行了确认，血清IgE水平（2.6 - 5500 ng/ml）与体内IgE分子/嗜碱性粒细胞数量（6000 - 600000）具有良好的相关性（rs = 0.95，P < 0.001）。通过将嗜碱性粒细胞与富含IgE的血清（15 μg/ml）孵育、在pH 3.7下从细胞中定量洗脱IgE并通过直接放射免疫吸附试验测量洗脱的IgE，来监测每个嗜碱性粒细胞上IgE受体的总数。用15 nM IgE（3 μg/ml）使每个供体的受体达到饱和。体内被占据的受体比例与血清IgE相关（rs = 0.84，P < 0.001），而受体的平均结合常数与血清IgE无关，范围为7.1×10⁸/M至2.8×10¹⁰/M，平均为7.7×10⁹/M。出乎意料的是，每个嗜碱性粒细胞上IgE受体的总数与血清IgE水平密切相关（rs = 0.92，P < 0.001）。因此，要么血清IgE与嗜碱性粒细胞IgE受体数量之间存在遗传关联，要么更有可能的是，受体数量受血清IgE浓度调节。
