大鼠肝脏中的血栓烷合成系统。

Thromboxane synthesizing system in rat liver.

作者信息

Mahmud I, Fukui N, Miura Y, Brandenberger H

出版信息

Prostaglandins Leukot Med. 1984 Dec;16(3):371-7. doi: 10.1016/0262-1746(84)90193-8.

Abstract

Thromboxane B2 (TXB2), along with other primary prostaglandins, was synthesized when rat liver microsomes were incubated with radioactive arachidonic acid. TXB2 was identified directly by chemical ionization mass spectrometry and indirectly by using specific inhibitors of TX synthetase, viz., imidazole and OKY-1555 ((E)-3(4-(3-pyridyl-methyl) phenyl)-2 methyl acrylic acid HCl). The supernatant fraction obtained after centrifugation at 105,000 X g for 60 min contained a possible regulatory component that suppressed thromboxane synthesis. The regulatory influence is lost after partial hepatectomy.

摘要

当大鼠肝微粒体与放射性花生四烯酸一起温育时，血栓素B2（TXB2）与其他主要前列腺素一起合成。通过化学电离质谱直接鉴定TXB2，并通过使用TX合成酶的特异性抑制剂，即咪唑和OKY-1555（（E）-3（4-（3-吡啶基甲基）苯基）-2-甲基丙烯酸盐酸盐）间接鉴定。在105,000×g离心60分钟后获得的上清液部分含有一种可能抑制血栓素合成的调节成分。部分肝切除术后调节作用丧失。