Simpson M A, Gozzo J J
J Immunol Methods. 1978;21(1-2):159-65. doi: 10.1016/0022-1759(78)90232-6.
An assay has been developed in which the degree of in vitro spleen cell-mediated sheep red blood cell (SRBC) hemolysis is quantitated spectrophotometrically (QHS). Immune mouse spleen cells, SRBC and guinea pig complement were incubated at 37 degrees C for 1 h in buffer, centrifuged, and the supernatant solutions read at 413 nm. The greatest degree of cell-mediated SRBC hemolysis was observed in day +5 harvested spleens following a day 0, 5% SRBC i.p. injection. In the assay system, it was found that a stimulator SRBC dose of 0.2% resulted in maximal stimulation with little non-specific hemolysis. This assay is presented as an alternative to the well established plaque forming and rosette assays.
已开发出一种检测方法，其中通过分光光度法定量体外脾细胞介导的绵羊红细胞（SRBC）溶血程度（QHS）。将免疫小鼠脾细胞、SRBC和豚鼠补体在缓冲液中于37℃孵育1小时，离心，然后在413nm处读取上清液。在第0天腹腔注射5% SRBC后，于第5天收获的脾脏中观察到最大程度的细胞介导的SRBC溶血。在该检测系统中，发现刺激剂SRBC剂量为0.2%时可产生最大刺激，且非特异性溶血很少。该检测方法作为成熟的噬斑形成和玫瑰花结检测方法的替代方法被提出。
