Institute of Health and Environmental Medicine, Tianjin, 300050, China.
By-Health Nutrition and Health Research Center, 510620, China.
J Clin Biochem Nutr. 2014 Jul;55(1):7-10. doi: 10.3164/jcbn.13-70. Epub 2014 Jul 1.
Aging weakened innate and adaptive immunity both quantitatively and qualitatively. Some components in propolis could stimulate immune function in young animals or cultured immune cells in vitro. Few studies had been carried out in the aged. The present study was to evaluate the effects of Brazilian green propolis supplementation on the immunological parameters in aged mice. Eighty Kunming mice, aged 15-18 months, were randomly assigned to the control and three experimental groups supplemented with different doses (83.3, 157.4 and 352.9 mg/kg.bw respectively) of Brazilian green propolis. The experiment lasted for 4 weeks. Contents of total polyphenol, flavonoid, cinnamic acid and artepillin-C in Brazilian green propolis were analyzed. Splenic NK cytotoxic, T lymphocyte proliferation and antibody generation cells, as well as the phagocytosis of peritoneal macrophages, ear swelling, and serum contents of IgG, IgM, hemolysin and cytokines were measured. After 4 weeks of treatment, the phagocytosis of peritoneal macrophages was enhanced in 157.4 mg/kg and 352.9 mg/kg groups. Ear swelling increased in all propolis treatmented groups. Antibodies specific to sheep erythrocytes were higher in the groups receiving 157.4 and 352.9 mg/kg.bw than that of control group. IgG level dramatically increased in the groups receiving 83.3 and 157.4 mg/kg.bw in comparison to the control group. These results indicate that administration of Brazilian green propolis have a positive effect on innate and adaptive immunity in aged mice.
衰老是机体固有免疫和适应性免疫均发生数量和质量下降的过程。某些蜂胶成分可刺激年轻动物或体外培养的免疫细胞的免疫功能。目前，关于蜂胶对衰老机体免疫功能影响的研究较少。本研究旨在评估巴西绿蜂胶补充剂对老年小鼠免疫参数的影响。将 80 只 15-18 月龄昆明种小鼠随机分为对照组和 3 个实验组，分别灌胃给予不同剂量（83.3、157.4 和 352.9 mg/kg·bw）的巴西绿蜂胶。实验持续 4 周。分析巴西绿蜂胶中总多酚、黄酮、肉桂酸和阿魏酸的含量。检测脾 NK 细胞细胞毒性、T 淋巴细胞增殖和抗体生成细胞、腹腔巨噬细胞吞噬功能、耳肿胀度以及血清 IgG、IgM、溶血素和细胞因子含量。经过 4 周的治疗，157.4 和 352.9 mg/kg 组腹腔巨噬细胞的吞噬功能增强。所有蜂胶处理组的耳肿胀度均增加。接受 157.4 和 352.9 mg/kg·bw 剂量的实验组绵羊红细胞特异性抗体高于对照组。与对照组相比，接受 83.3 和 157.4 mg/kg·bw 剂量的实验组 IgG 水平显著升高。这些结果表明，巴西绿蜂胶的给予对老年小鼠固有免疫和适应性免疫具有积极影响。
