从细胞壁酶裂解物制备的口腔放线菌新抗原。

Novel antigens of oral Actinomyces species prepared from a cell wall enzyme lysate.

作者信息

Hamada S, Okahashi N, Kimura S, Imanishi H, Koga T, Kawata S, Michalek S M, McGhee J R

出版信息

Jpn J Med Sci Biol. 1982 Aug;35(4):171-81. doi: 10.7883/yoken1952.35.171.

DOI:10.7883/yoken1952.35.171

Abstract

Purified cell walls of Actinomyces viscosus and Actinomyces naeslundii were solubilized by the enzymatic action of Ml N-acetylmuramidase. Soluble cell wall carbohydrate antigens were purified by column chromatography. A purified antigen preparation (fraction C) from A. viscosus ATCC 19246 was found to contain deoxyhexoses (rhamnose and 6-deoxytalose), hexosse (galactose, glucose and mannose), and a peptidoglycan component. This preparation gave a single precipitin band against antiserum to homologous whole cells. Similar antigen preparations were purified from Ml enzyme lysate of A. viscosus SK4 and A. naeslundii ATCC 12104 cell walls.

摘要

粘性放线菌和内氏放线菌的纯化细胞壁通过Ml N - 乙酰胞壁酸酶的酶促作用溶解。可溶性细胞壁碳水化合物抗原通过柱色谱法纯化。发现来自粘性放线菌ATCC 19246的纯化抗原制剂（C级分）含有脱氧己糖（鼠李糖和6 - 脱氧塔罗糖）、己糖（半乳糖、葡萄糖和甘露糖）以及肽聚糖成分。该制剂针对同源全细胞抗血清产生单一沉淀带。从粘性放线菌SK4和内氏放线菌ATCC 12104细胞壁的Ml酶裂解物中纯化出了类似的抗原制剂。