Macnaughton M R, Madge M H
J Gen Virol. 1978 Jun;39(3):497-504. doi: 10.1099/0022-1317-39-3-497.
The genomic RNA of human coronavirus strain 229E (HCV 229E) migrated on polyacrylamide gels as a single peak with a mol. wt. of 5.8 X 10(6). Denaturation of the genome with formaldehyde did not alter its electrophoretic mobility, which suggests that the HCV 229E genome is a single-stranded molecule. At least 30% of the genomic RNA was shown to contain covalently attached polyadenylic acid [poly(A)]sequences by binding the RNA to an oligo(dT)-cellulose column. These poly(A) tracts were shown to be about 70 nucleotides in length by measuring the resistance to digestion of HCV 229E RNA with pancreatic and T1 RNases. Finally, the genomic RNA was shown to terminate at or near the 3'-terminus on the basis of its susceptibility to polynucleotide phosphorylase.
人冠状病毒229E株（HCV 229E）的基因组RNA在聚丙烯酰胺凝胶上迁移时呈现为一个单一峰，分子量为5.8×10⁶。用甲醛使基因组变性并未改变其电泳迁移率，这表明HCV 229E基因组是单链分子。通过将RNA与寡聚（dT）-纤维素柱结合，显示至少30%的基因组RNA含有共价连接的聚腺苷酸[poly（A）]序列。通过测量HCV 229E RNA对胰核糖核酸酶和T1核糖核酸酶消化的抗性，表明这些聚（A）区段长度约为70个核苷酸。最后，基于基因组RNA对多核苷酸磷酸化酶的敏感性，表明其在3'-末端或附近终止。
