Gerrard T L, Jurgensen C H, Fauci A S
Cell Immunol. 1983 Dec;82(2):394-402. doi: 10.1016/0008-8749(83)90172-7.
A differential role for DR antigens on monocytes in antigen-stimulated as opposed to mitogen-stimulated human lymphocyte responses has been observed. A monoclonal anti-DR antibody used to treat monocytes caused inhibition of antigen-induced T-cell responses and of T-cell-dependent B-cell responses. However, anti-DR antibody treatment of monocytes did not inhibit mitogen-induced responses. Anti-DR treatment of monocytes did not induce suppression, as antigen-induced responses could be reconstituted with untreated monocytes. Anti-DR treatment of monocytes did not merely block interleukin 1 (IL-1) secretion since addition of IL-1 could not restore antigen-induced responses. Monoclonal anti-DR antibody did not directly inhibit monocyte secretion of IL-1. DR-negative monocytes, selected by antibody and complement, could not present antigen, even though they were capable of secreting IL-1. Thus, this monoclonal anti-DR antibody sterically blocks antigen presentation by monocytes without induction of suppression or inhibition of IL-1 secretion. Monocyte DR antigens appear essential for stimulation of antigen-induced responses, but DR antigens on monocytes may not be essential for mitogen-stimulated responses and do not appear to be related to the ability of monocytes to secrete IL-1.
已观察到，在抗原刺激而非丝裂原刺激的人类淋巴细胞反应中，单核细胞上的DR抗原具有不同作用。一种用于处理单核细胞的单克隆抗DR抗体可抑制抗原诱导的T细胞反应以及T细胞依赖性B细胞反应。然而，用抗DR抗体处理单核细胞并不会抑制丝裂原诱导的反应。对单核细胞进行抗DR处理不会诱导抑制作用，因为用未处理的单核细胞可重建抗原诱导的反应。对单核细胞进行抗DR处理并非仅仅阻断白细胞介素1（IL-1）的分泌，因为添加IL-1无法恢复抗原诱导的反应。单克隆抗DR抗体不会直接抑制单核细胞分泌IL-1。通过抗体和补体选择的DR阴性单核细胞无法呈递抗原，尽管它们能够分泌IL-1。因此，这种单克隆抗DR抗体在空间上阻断了单核细胞的抗原呈递，而不会诱导抑制作用或抑制IL-1的分泌。单核细胞DR抗原似乎对抗原诱导反应的刺激至关重要，但单核细胞上的DR抗原对丝裂原刺激的反应可能并非必不可少，而且似乎与单核细胞分泌IL-1的能力无关。
