Sørensen S, Trentemøller S
Oncodev Biol Med. 1983;4(5):351-61.
The major component of the pregnancy-specific beta 1-glycoprotein (SP1) was purified from pregnancy sera by ammonium sulphate precipitation (1.95 mol/l), anion-exchange chromatography at pH 7.0, positive affinity chromatography, negative affinity chromatography, anion-exchange chromatography at pH 5.0 and, finally, concentration by ultrafiltration or lyophilization. Essentially, the first anion-exchange chromatography separated the major component from three minor components, termed SP1 (gamma), SP1 (alpha 2(2)) and SP1 (alpha 2(1)) on the basis of their electrophoretic mobilities. The yield was 3-6% and the purity about 98-99%. A highly sensitive radioimmunoassay to determine SP1 was developed (detection limit, about 0.5 microgram/l). By using a commercial antiserum against SP1, the slopes of the dose-response curves for purified SP1, a reference preparation from the International Agency for Cancer Research, a commercial SP1 standard, and two of three minor SP1 components were all similar to that of a pool of pregnancy sera, whereas the third minor component, SP1 (gamma), showed a marked difference in slope.
妊娠特异性β1-糖蛋白（SP1）的主要成分通过以下步骤从妊娠血清中纯化得到：硫酸铵沉淀（1.95 mol/l）、pH 7.0的阴离子交换色谱、正亲和色谱、负亲和色谱、pH 5.0的阴离子交换色谱，最后通过超滤或冻干浓缩。基本上，第一次阴离子交换色谱根据其电泳迁移率将主要成分与三种次要成分分离，这三种次要成分分别称为SP1（γ）、SP1（α2(2)）和SP1（α2(1)）。产率为3 - 6%，纯度约为98 - 99%。开发了一种用于测定SP1的高灵敏度放射免疫分析方法（检测限约为0.5微克/升）。使用针对SP1的商业抗血清时，纯化的SP1、国际癌症研究机构的参考制剂、商业SP1标准品以及三种次要SP1成分中的两种的剂量反应曲线斜率均与妊娠血清池的相似，而第三种次要成分SP1（γ）的斜率则有明显差异。
