Hough D W, Miller N I, Maddison P J
Immunol Lett. 1983 Mar;6(3):123-7. doi: 10.1016/0165-2478(83)90093-7.
DNA-dependent RNA polymerase was partially purified from wheat germ extract and tested for inhibition by antinuclear autoantibodies from the sera of patients with connective tissue diseases. The enzyme was inhibited by anti-DNA and by autoantibodies to the nuclear ribonucleoprotein nRNP. Autoantibodies to other ribonucleoproteins (Sm, Ro, La) did not cause inhibition. The enzyme preparation was shown to contain material with Ro and La antigenic activity but there was no nRNP or Sm detectable by immune precipitation. Previous work [3] has shown inhibition of prokaryotic (E. coli) RNA polymerase by anti-DNA, and our results show that the eukaryotic enzyme, in this case from wheat germ, is also inhibited. The results are consistent with the suggestion that inhibition by anti-DNA is due to template masking. Inhibition of RNA polymerase by antibodies to cellular ribonucleoprotein suggests that the antigen is in some way associated with the activity of the enzyme.
从麦胚提取物中部分纯化了依赖DNA的RNA聚合酶，并检测了结缔组织病患者血清中的抗核自身抗体对其的抑制作用。该酶受到抗DNA抗体以及针对核糖核蛋白nRNP的自身抗体的抑制。针对其他核糖核蛋白（Sm、Ro、La）的自身抗体未产生抑制作用。酶制剂显示含有具有Ro和La抗原活性的物质，但通过免疫沉淀未检测到nRNP或Sm。先前的研究[3]表明抗DNA可抑制原核生物（大肠杆菌）RNA聚合酶，我们的结果表明真核酶（此处为来自麦胚的酶）也受到抑制。这些结果与抗DNA抑制是由于模板掩盖这一观点一致。针对细胞核糖核蛋白的抗体对RNA聚合酶的抑制表明该抗原在某种程度上与酶的活性相关。
