Ow K T, Abandowitz H M, Sarfaty G A, Nash A R
Endocrinol Exp. 1983 Mar;17(1):69-77.
In contrast to the in vivo action of sex steroids the steroidal hormones dihydrotestosterone (DHT) and estradiol (E2) produced a marked variable response on mitogen-induced lymphoblastogenesis. DHT and E2 at concentrations of 10(-11) mol l-1 incubated with mononuclear cell fraction in the presence of mitogens PHA, Con A and PWM caused inhibition, stimulation or no effect. Separation of T and B cell fractions and incubation with DHT and E2 likewise produced variations in response. However, dexamethasone (DEX) strongly inhibited proliferation of all cell fractions, particularly at the higher steroid concentrations. These results suggest that the reported effects of in vivo administered sex hormones on the immune system might possibly be mediated by a precursor cell of peripheral blood mononuclear cells, and that the in vitro system may not be a reproducible test for determining an individual's immunoendocrine status.
与性类固醇的体内作用相反，甾体激素双氢睾酮（DHT）和雌二醇（E2）对有丝分裂原诱导的淋巴细胞生成产生了明显的可变反应。在有丝分裂原PHA、Con A和PWM存在的情况下，浓度为10(-11) mol l-1的DHT和E2与单核细胞组分一起孵育会导致抑制、刺激或无作用。分离T细胞和B细胞组分并与DHT和E2一起孵育同样会产生反应差异。然而，地塞米松（DEX）强烈抑制所有细胞组分的增殖，尤其是在较高的类固醇浓度下。这些结果表明，体内施用性激素对免疫系统的报道效应可能是由外周血单核细胞的前体细胞介导的，并且体外系统可能不是确定个体免疫内分泌状态的可重复测试。
