Souvannavong V, Rimsky L, Adam A
Biochem Biophys Res Commun. 1983 Jul 29;114(2):721-8. doi: 10.1016/0006-291x(83)90840-9.
The involvement of macrophages in the adjuvanticity of N-acetyl-muramyl-L-alanyl-D-isoglutamine (MDP) has been examined. The stimulation of the in vitro primary immune response to sheep red blood cells (SRBC) has been studied, because it is known that macrophages cooperate through the mediation of soluble compounds for the induction of the anti-SRBC response. The cultures depleted of macrophages by passing spleen cells on Sephadex G-10 were unable to give any response to SRBC. Their immune responsiveness was fully restored by the addition of either Interleukine 1 (IL 1) obtained from P388D1 cells or a factor able to replace macrophages (FRM) obtained from resident peritoneal macrophages. MDP alone, at any dose, was unable to induce any response in such macrophage depleted cultures, but it was able to enhance the antibody response of these cultures reconstituted with monokines, with the same characteristics in dose effect and timing dependence than in whole spleen cells.
已对巨噬细胞在N-乙酰-胞壁酰-L-丙氨酰-D-异谷氨酰胺（MDP）佐剂活性中的作用进行了研究。对绵羊红细胞（SRBC）的体外初次免疫应答刺激进行了研究，因为已知巨噬细胞通过可溶性化合物的介导来协同诱导抗SRBC应答。通过在葡聚糖凝胶G-10上通过脾细胞而耗尽巨噬细胞的培养物对SRBC无任何应答。通过添加从P388D1细胞获得的白细胞介素1（IL 1）或从驻留腹膜巨噬细胞获得的能够替代巨噬细胞的因子（FRM），其免疫反应性得以完全恢复。单独的MDP在任何剂量下均无法在此类巨噬细胞耗尽的培养物中诱导任何应答，但它能够增强用单核因子重构的这些培养物的抗体应答，其剂量效应和时间依赖性特征与全脾细胞中的相同。
