Zuniga M C, Malissen B, McMillan M, Brayton P R, Clark S S, Forman J, Hood L
Cell. 1983 Sep;34(2):535-44. doi: 10.1016/0092-8674(83)90386-0.
Two mutants of the class I gene encoding the H-2Ld transplantation antigen have been constructed. In one mutant the cytoplasmic domain of the class I molecule has been altered by deletion of 24 of the 31 C-terminal residues, and in the second the C-terminal 25 residues of the cytoplasmic domain have been replaced with a unique sequence of 19 amino acids. These mutant class I genes have been transferred into mouse L cells by DNA-mediated gene transfer. Both mutant genes are expressed at normal levels on the cell surface, and they have charge properties and sizes consistent with the introduced alterations. These mutant Ld molecules can serve as target antigens for allogeneic cytotoxic T cells and as restricting elements for virus-specific cytotoxic T cells. These results show that the 24 residues replaced or deleted from the carboxy terminus of the class I molecule are not required for its transport to or integration in the plasma membrane, nor for its function as a target antigen or a restricting element during T-cell-mediated cytotoxicity.
编码H-2Ld移植抗原的I类基因的两个突变体已构建成功。在一个突变体中，I类分子的胞质结构域因31个C末端残基中的24个缺失而发生改变，在另一个突变体中，胞质结构域的C末端25个残基被19个氨基酸的独特序列所取代。这些突变的I类基因已通过DNA介导的基因转移导入小鼠L细胞。两个突变基因均在细胞表面以正常水平表达，并且它们的电荷性质和大小与引入的改变一致。这些突变的Ld分子可作为同种异体细胞毒性T细胞的靶抗原以及病毒特异性细胞毒性T细胞的限制元件。这些结果表明，从I类分子羧基末端替换或缺失的24个残基对于其转运至质膜或整合入质膜，以及在T细胞介导的细胞毒性过程中作为靶抗原或限制元件的功能而言并非必需。
