Brockbank K G, Ploemacher R E, van Peer C M
Mech Ageing Dev. 1983 May;22(1):11-21. doi: 10.1016/0047-6374(83)90003-9.
We determined the number of fibroblastoid progenitors (fibroblastoid colony-forming units, CFU-F) in femurs and spleens derived from (CBA X C57BL)F1 mice of different ages. The femoral CFU-F population size increased from 350 at 1 week of age and plateaued at approximately 1900 CFU-F at 8 weeks of age. The mean incidence of CFU-F per 10(6) femoral marrow nucleated cells decreased from 82 at 8 weeks of age to 55 at 70 weeks of age; however, due to an increase in femur cellularity, there was no decrease in the CFU-F population size. The splenic CFU-F population decreased from 1700 at 1 week of age to 180 at 8 weeks of age; no further change was observed in mice up to 70 weeks' old. Analysis of colony-stimulating activity production by fibroblastoid colonies derived from young (6 weeks) and aged (70 weeks) mouse femoral marrow demonstrated no difference. These results indicate that there is no change in CFU-F numbers or fibroblastoid cell colony-stimulating activity production associated with the age-related increase in hemopoietic organ cellularity and hemopoietic progenitor content observed in this mouse strain. There were, however, major changes in the CFU-F population sizes during development of both femoral marrow and spleen in the first 2 months after birth.
我们测定了来自不同年龄的（CBA×C57BL）F1小鼠的股骨和脾脏中成纤维细胞样祖细胞（成纤维细胞样集落形成单位，CFU-F）的数量。股骨CFU-F群体大小从1周龄时的350增加，在8周龄时稳定在约1900 CFU-F。每10⁶个股骨骨髓有核细胞中CFU-F的平均发生率从8周龄时的82降至70周龄时的55；然而，由于股骨细胞数量增加，CFU-F群体大小没有减少。脾脏CFU-F群体从1周龄时的1700降至8周龄时的180；在70周龄的小鼠中未观察到进一步变化。对来自年轻（6周）和老年（70周）小鼠股骨骨髓的成纤维细胞样集落的集落刺激活性产生的分析表明没有差异。这些结果表明，在该小鼠品系中观察到的与造血器官细胞数量和造血祖细胞含量的年龄相关增加相关的CFU-F数量或成纤维细胞样细胞集落刺激活性产生没有变化。然而，在出生后的头2个月，股骨骨髓和脾脏发育过程中CFU-F群体大小发生了重大变化。
