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接种物生长阶段对微量稀释药敏试验的影响。

Influence of inoculum growth phase on microdilution susceptibility tests.

作者信息

Barry A L, Badal R E, Hawkinson R W

出版信息

J Clin Microbiol. 1983 Sep;18(3):645-51. doi: 10.1128/jcm.18.3.645-651.1983.

Abstract

Two types of commercially available microdilution trays were inoculated with log-phase cultures, stationary-phase cultures, and direct suspensions of 18- to 24-h colonies, with essentially comparable results. Direct pick-up of colonies was also tested with two Prompt inoculation systems (3M Co.); they were found to be convenient and reliable methods for standardizing inocula without preincubation in broth and without turbidity adjustment.

摘要

两种市售的微量稀释板接种对数期培养物、稳定期培养物以及18至24小时菌落的直接悬液,结果基本相当。还使用两种快速接种系统(3M公司)测试了直接挑取菌落的方法;发现它们是在无需肉汤预培养和无需调整浊度的情况下标准化接种物的便捷可靠方法。

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